Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810105226
Authors: Garg, Shivanshu
Advisor: Singh, Nageswer
Title: SILENCING OF F-BOX KELCH GENE FOR VIRUS RESISTANCE STUDIES IN MODEL PLANT NICOTIANA BENTHAMIANA
Publisher: CSKHPKV, Palampur
Language: en
Type: Thesis
Pages: 50
Agrotags: null
Keywords: Nicotiana benthamiana
Abstract: In the present investigation entitled ―Silencing of F-Box kelch gene for virus resistance studies in model plant Nicotiana benthamiana‖, construct has been prepared for generating the silent lines of the mentioned plant. F-Box kelch is an important protein that associates with the viral proteins and that the protein is an important factor that helps in the establishment of viral infection. A conserved portion of the gene was amplified in sense and antisense orientation and in a sequential manner it was cloned into the silencing vector pSAT4. The construct development was ascertained with the help of restriction digestion and sequence determination. The construct is ready to be transformed into plants. For the biochemical analysis, purified Cucumber mosaic virus (CMV) was inoculated at their 3-4 leaf stage and infection was confined by symptom development. Both infected and healthy plants were subjected to biochemical analysis of the phenolic compounds (total phenols, simple phenols, Ortho-dihydric phenols and bound phenols), alkaloids and total chlorophyll and carotenoids. It was observed that the total phenol content was increased in the infected plant in comparison to the healthy leaves. However, total chlorophyll and carotenoids were reduced in infected plants as compared to healthy leaves. This is expected as virus infection leads to loss of chlorophyll as reflected by mosaic symptoms that the virus produces.
Subject: Biochemistry
Theme: Silencing of F-Box kelch gene for virus resistance
These Type: M.Sc
Issue Date: 2019
Appears in Collections:Theses

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