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|Title:||ISOLATION AND PURIFICATION OF LECTINS FROM VIGNA SPECIES|
|Abstract:||In the present investigation, seeds of five Vigna species were investigated for hemagglutinating activity against human and rabbit erythrocytes. The seed extracts of Vigna mungo and Vigna radiata showed agglutination activity with human as well as rabbit erythrocytes, whereas Vigna unguiculata, Vigna umbellata and Vigna angularis exhibited agglutination only with trypsinized rabbit erythrocytes. Among the various tested sugars, agglutination activity was best inhibited by D-galactose. Vigna radiata extract exhibited a higher level of agglutination with rabbit erythrocytes and was selected for isolation and purification of lectin. Lectin was purified by employing a protocol that entailed ion exchange, gel filtration and affinity chromatography with 192.47 fold purification and 73.14 (HU/mg) specific activity. Under reducing conditions purified lectin was found to be a monomer with 25.0kDa molecular weight. Maximum agglutination activity of purified lectin was observed at pH 5.0 with (98%) stability and maintained steadily between pH 6-9. Maximum agglutination activity was observed at 370C (68.76%) and minimum at 650C where agglutination activity was completely lost. Agglutination activity was readily inhibited by D-galactose at 3.12mM. Lectins, due to various biological properties have a potential in pest control and plant defense. Their high level expression can confer resistance to a wide range of insects and could be exploited for crop improvement.|
|Theme:||Purification of lectins from Vigna species|
|Appears in Collections:||Theses|
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