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Advisor: H. K. Dikshit
Title: Tagging gene(s) for resistance to wilt in lentil (Lens culinaris Medikus subsp. culinaris)
Language: en_US
Type: Thesis
Agrotags: null
Keywords: Fusarium wilt, Gene, Lentil, Mapping Population and Markers
Abstract: Fusarium wilt caused by Fusarium oxysporum f.sp. lentis Vasu. and Srini. is a serious disease of lentil (Lens culinaris Medikus), causing severe yield losses worldwide and it is a major disease in Central India. The disease is soil borne causing huge losses and development of wilt resistant varieties is most effective means of controlling this disease. Highly resistant sources of wilt in lentil have not been reported from the Indian lentil breeding programme. Ninety three lentil accessions including twelve varieties, six ICARDA germplasm lines and seventy five advanced breeding lines were evaluated in field and controlled conditions against wilt. Among which six genotypes IG 69549, IG70238, IG71487, ILL10916, ILL10921 and ILL10965 were identified as resistant in wilt sick plot as well as greenhouse condition. The germplasm L9-12 as wilt susceptible parent and ILL10965 as wilt resistance parent were used to develop mapping population. The inheritance of wilt-resistance gene in lentil was investigated in F2 mapping population (L9-12 × ILL10965) exhibited 3:1 ratio of resistance and susceptible plants and F2:3 mapping population plants exhibited 1:2:1 ratio for resistance, segregating and susceptible reaction respectively. It confirmed that wilt resistance in lentil is dominant over susceptibility without any maternal effect and controlled by a single dominant gene. Both wilt resistance gene inheritance and marker segregation was confirmed by Chi-square test, which fitted well the 3:1 ratio. Three hundred two SSR markers were surveyed for the parental polymorphism, in which three SSRs viz. PBALC233, PBALC1409 and PBALC203 were found polymorphic between parents as well as resistance and susceptible bulks. Linkage analysis showed two SSR markers, PBALC203 and PBALC1409 flanking the wilt resistance gene at 8.2 cM and 9.4 cM distance, respectively. Further, PBALC233 was also found present on the same linkage group at a distance of 10.2 cM from PBALC1409. Keywords : Fusarium wilt, Gene, Lentil, Mapping Population and Markers
Description: t-9960
Subject: Genetics and Plant Breeding
Theme: Tagging gene(s) for resistance to wilt in lentil (Lens culinaris Medikus subsp. culinaris)
These Type: Ph.D
Issue Date: 2018
Appears in Collections:Theses

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