Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810094242
Authors: BHATTACHARJEE, PANCHAAL
Advisor: SAKTHIVEL, T.
Naik, Nagesh
I. N., DOREYAPPA GOWDA
C., ASWATH
K. H, NATARAJA
Title: STUDIES ON VEGETATIVE PROPAGATION IN RAMBUTAN (Nephilium lappaceum L.)
Publisher: K.R.C, COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)
Language: en
Type: Thesis
Pages: 137
Agrotags: null
Keywords: RAMBUTAN, VEGETATIVE PROPAGATION
Abstract: Being a crop of exotic origin, the area expansion of rambutan is significantly relied on the supply of quality planting material. Studies on Vegetative propagation in Rambutan (Nephilium lappaceum L.) were carried out in order to standardize suitable vegetative propagation technique (viz. budding, air layering, grafting) and a micro propagation protocol for in vitro rambutan rootstock multiplication. Theexperiments were conducted at two different locations during 2017-18.Investigations onbudding,air layering, grafting techniques in rambutan was carried out at Central Horticultural Experiment Station (CHES), Chettalli, Kodagu, Karnataka. Whereas, the micro-propagation experiments of rambutan was carried out in the plant tissue culture laboratory at ICAR-IIHR, Bengaluru, Karnataka. Patch budding was the best among the three different budding methods viz. patch, forkert and chip budding carried out with significant ly higher rate of budding success (70.00 %), minimum days taken for sprouting (36.86 days) andsignificantly lowest rate of mortality (6.28 %).In air layering, among the two different rooting media substrates used coir fibre was better than coco peatin terms of success in rooting (22.50 %), survival (53.12 %).Amid of the different concentration of IBA was tried, significantly better response was observed in treatment of IBA 2500ppm.Within the interaction treatments, M1T1-Coir fibre with IBA 2500ppm was significantly the best treatment with highest percent success of rooting (37.21%), higher numbers of primary roots (13.33), and higher percentage of survival (87.50 %). Approach grafting method was superior among different methods of grafting studied with maximum rate of graft success (72.86 %), minimum days required for sprouting (46.29 days) and higher number of sprouted bud (4.29) along with a very low mortality rate (5.87 %). In micro-propagation studies9 different types of rambutan explants were tried, single nodal segments from both lab grown current season seedlings and one year old seedlings showed better survival rate of 46.67 % and 43.33 %, respectively. Surface sterilization of the explants was effectively achieved when treated with Carbendazim 50 WP (2 %, 30min) + Gentamicin (2000ppm, 20min) + Mercuric Chloride (0.1 %, 5min) with a lower rate of contamination (31.67 %), highersurvival rate (61.67 %). Ascorbic acid (0.05 %) + 0.5 % PVP + 2 % Sucrose proved to be the best treatment to reduce phenolic browning (10.00 %), with a higher (85.55 %) rate of survival.Best media for shoot initiation was found to be WPM + BAP (2mg/l) in both nodal explants from lab grown current season seedling and one year old seedling sources, exhibiting 91.67 % and 86.67 % of survival respectively with least numbers of days (9.33 and 9 days respectively) required to initiate shoot growth.GA3 was found effective for the purpose of shoot elongation at a concentration of 2 mg/l,the shoots were significantly longer (3.18 cm) with highest mean diameter of shoot (0.46cm).Best in vitro rooting was achieved with MS + IBA (4mg/l) + IAA (4mg/l), in terms of highest rooting (78.33 %) with lesser days required for root initiation (38.67 days) and rooted plantlets were successfully hardened by transferring them to plastic cups containing coco peat.
Description: TH-649
Subject: Fruit Science
Theme: STUDIES ON VEGETATIVE PROPAGATION IN RAMBUTAN (Nephilium lappaceum L.)
Research Problem: STUDIES ON VEGETATIVE PROPAGATION IN RAMBUTAN (Nephilium lappaceum L.)
These Type: M.Sc
Issue Date: 2018-08-30
Appears in Collections:Theses

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