Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810093364
Authors: UZMA MEHRAJ
Advisor: Sapna Panwar
Title: In vitro mass multiplication of doubled haploid line of marigold (Tagetes erecta L.) derived through ovule culture
Publisher: DIVISION OF FLORICULTURE AND LANDSCAPING ICAR - INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI
Language: en_US
Type: Thesis
Agrotags: null
Keywords: In vitro mass multiplication of doubled haploid line of marigold (Tagetes erecta L.) derived through ovule culture
Abstract: Marigold (Tagetes spp.) is one of the most popular flower crop grown throughout the world. In India, marigold ranks first in area and production of loose flower crops. It is gaining popularity among flower growers on account of its free flowering habit, short duration, attractive colour, shape, keeping quality etc. Development of homozygous lines is essential for F1 hybrid development programme. Since marigold is highly cross pollinated crop therefore, traditional way of homozygous line development will take 8- 9 generations of continuous selfing. Hence, production of doubled haploids (DHs) is required to produce the homozygous lines in a single generation. Large scale multiplication of doubled haploids is required for commercial use. Hence, arise the need of development of protocol for in vitro mass multiplication of doubled haploid lines in marigold. The in vitro regenerated plants show high degree of mortality when taken to field conditions so there is a need to standardize an efficient protocol for ex vitro hardening. Clonal fidelity of in vitro multiplied plantlets needs to be assessed through molecular markers for testing their genetic stability. Hence, the present study was an initiative to develop a reliable and efficient protocol for in vitro mass multiplication of doubled haploid line of African marigold variety Local Orange derived through ovule culture. Among pre-treatments of leaves, treatment (T4) - Bavistin (0.2%) + Ridomil (0.2%) + 8-HQC (200 mg/l) for 60 minutes followed by HgCl2 for 3 minutes resulted in minimum contamination and maximum survival of explant. Surface sterilization of leaves with treatment T1 - HgCl2 (0.1%) for 3 minutes reduced microbial contamination and simultaneously increased the survival percentage. Three portions of leaf explant namely tip portion, middle portion and basal portion of doubled haploid line were used for regeneration. Treatment combination (T2) - MS + BAP (0.5 mg/l) + NAA (0.25 mg/l) showed maximum regeneration in all the three portions of leaf explant of doubled haploid line. Among the different leaf explant portions, basal portion was found best which resulted in maximum success and was found the optimum source of explant for mass multiplication of doubled haploid line. Treatment (T3) - MS +BAP (0.5 mg/l) + NAA (0.25 mg/l) + Putrescine (50.0 mg/l) was found best for morphogenesis and multiple shoot emergence. In rhizogenesis, treatment T1 (½MS+IBA 0.5 mg/l) was found best in respect of early rooting, rooting percentage and oth
Description: t-9874
Subject: Horticulture
Theme: In vitro mass multiplication of doubled haploid line of marigold (Tagetes erecta L.) derived through ovule culture
These Type: M.Sc
Issue Date: 2018
Appears in Collections:Theses

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