Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810093063
Authors: Syed, Aalia Mushtaq
Advisor: Irfan Ahmad
Title: Genetic Characterization of Saprolegnia spp. from trout in hatcheries of Kashmir
Publisher: SKUAST Kashmir
Language: en
Type: Thesis
Agrotags: Hyphae
Parasitica
Trout
Fisheries Science
Keywords: Hyphae, Parasitica, Saprolegnia spp. and Trout
Abstract: The present study was conducted for “Genetic Characterization of Saprolegnia spp. from trout in hatcheries of Kashmir”. A total of 50 and 95 infected samples were taken during the present study from the two main trout farms i.e. Laribal trout farm and Kokernaag trout farm from October 2013 to March 2014. The infected fishes showed signs of lethargy, irritation, loss of appetite, haemorrhages at the base of fins and deep wounds at the sites of severe infection associated with cottony wool like tufts on both dorsal and ventral side of body. Wet mount from infected lesions showed masses of mature and immature sporangia filled with spores. Hyphae appeared profusely branched, aseptate and multinucleate. The colonies on fungal agar at 37°C appeared with cysts of long hairs, whitish in color that finally turned blackish. The morphological characteristic features of the fungus confirmed it as Saprolegnia. DNA was isolated from the infected sample by the Ke et al. (2008) protocol. A total of 29 DNA samples were extracted. Quality and quantity was checked by agarose gel electrophoresis (1%) and UV-spectrophotometer. Rapd PCR was used for the amplification. 8 primers were used and only two primers gave consistent results. The number of amplified DNA fragments obtained by these primers ranged from 380-1800bp. RAPD profiles obtained revealed negligible genetic variation among the isolates. Only one group could be established indicating that the group of closely related strains having high degree of homology is present. This is the first kind of work on Genetic characterization of Saprolegnia spp. from trout in hatcheries of Kashmir.
Description: M.F.Sc Thesis submitted to SKUAST Kashmir
Subject: Fisheries
Theme: Genetic Characterization of Saprolegnia spp. from trout in hatcheries of Kashmir
Research Problem: Genetic Characterization of Saprolegnia spp. from trout in hatcheries of Kashmir
These Type: M.Sc
Issue Date: 2015
Appears in Collections:Thesis

Files in This Item:
File Description SizeFormat 
Syed Aliya Mushtaq.pdf3.43 MBAdobe PDFThumbnail
View/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.