Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810092316
Authors: ANUSHA, Ch.
Title: IDENTIFICATION OF NOVEL SOURCES OF RESISTANCE AND THEIR MECHANISMS TO RICE GALL MIDGE, Orseolia oryzae (WOOD- MASON)
Publisher: PROFESSOR JAYASHANKAR TELANGANA STATE AGRICULTURAL UNIVERSITY
Citation: D 10,175
Language: en
Type: Thesis
Pages: 158p.
Agrotags: null
Keywords: IDENTIFICATION OF NOVEL SOURCES OF RESISTANCE AND THEIR MECHANISMS TO RICE GALL MIDGE, Orseolia oryzae (WOOD- MASON)
Abstract: In the present study, 52 rice germplasm and 38 pre-breeding lines developed from six crosses along with resistant checks, viz., Kavya, W1263, RP2068-18-3-5, Abhaya, Aganni and CR57MR1523 and TN1 as susceptible check were evaluated in a replicated trial against gall midge biotypes, GMB1 at IIRR greenhouse and under field conditions against GMB3 at RARS, Jagtial and GMB4M at RARS, Warangal during Kharif 2014 and 2015. Among 52 germplasm lines, five lines viz., CR2613-1-1-1-5-1, CR2615-1, JGL11470, JGL11727 and Sukaradidhan1 had nil damage against GMB1. Intivadlu found resistant against GMB3 and none of the lines were found resistant against GMB4M. Promising test lines when genotypically characterized by using Gm1, gm3, Gm4, Gm8 and Gm11 gene linked molecular markers for presence/absence of resistant gene(s), revealed in presence of Gm1 gene in Yerravadlu, Intivadlu and CR2603 with Gm1 markers, RM5526 and RM1328. CR2613-1-1-1-5-1 and CR2615-1 had Gm1 and Gm11 genes; JGL11727 had Gm1, gm3 and Gm11 genes; Sukaradidhan-1 had gm3 and Gm11 genes and JGL11470 had Gm1 gene, which are said to be the new sources of resistance for gall midge. In addition, in the present study Gm11 primer, (RM235) had amplified a novel allele in the germplasm viz., JGL11470, Kempudoddi, Yerravadlu, Intivadlu and CR2603. Among 38 pre-breeding lines from six crosses, 12 resistant lines from four crosses were identified against GMB1 under greenhouse conditions. Seven resistant pre-breeding lines were identified from two crosses against GMB3. Resistant lines against GMB1 and GMB3 showed HR- reaction and genotypically displayed Gm1 gene. None of the lines were found resistant against GMB4M under field conditions. Gene expression study was done with three Gm1 candidate genes viz., vonWillebrand factor type A domain (vWFA), Respiratory burst oxidase (RBO) and Cinnamoyl Co A reductase (CCA). The two breeding lines selected for this study were, the resistant line (RNR19880) and the most susceptible line, (RNR19886) from the cross MTU1010/JGL3855, TN1 as susceptible check and Kavya as resistant check. Of these, the Relative expression value (REV) of the vonWillebrand factor type A domain gene in RNR19880 was significantly higher (10.3 fold) as compared to the susceptible line, RNR19886 at 24 hours after infestation (HAI) (3.5 fold). The REV of Respiratory burst oxidase at 24 HAI was significantly low in R line RNR 19880 as compared to S line RNR19886; conversely at 120 HAI it was significantly high in the R line as compared to the expression in S line. REV of TN1 vs Kavya at 24 HAI was significant but fold change at 120 HAI was not significant. Cinnamoyl Co A reductase has not showed significant fold change in the selected lines. The study reveals that vWFA and RBO could be the possible genes implicated in imparting resistance in RNR19880 derived from MTU1010/JGL3855 against both the biotypes 1 and 3. From the present study identified resistance genes from germplasm lines can be used for pyramiding in the popular varieties of rice based on the geographical needs. Identification of phenotypically contrasting lines will be helpful for fine mapping of Gm1 gene and also useful for gene expression studies.
Subject: Entomology
Theme: Entomology
These Type: Ph.D
Issue Date: 2017
Appears in Collections:Theses

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