Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810090099
Authors: Rathod, A. D.
Advisor: Waskar, V. S.
Title: Occurrence, Characterization and Antibiogram Pattern of Pathogenic Listeria species
Publisher: MAFSU, Nagpur
Language: en
Type: Thesis
Pages: 117
Agrotags: null
Keywords: Veterinary Public, Health, Characterization, Antibiogram Pattern, Pathogenic, Listeria, species
Abstract: occurrence of pathogenic Listeria species, followed by characterization of isolates for antibiotic resistance. A total of 450 samples comprising milk, milk products, meat, meat products, aquatic food, water, vegetables and clinical samples (human and animal) were processed for the detection of Listeria species . The overall occurrence of Listeria spp in various samples was 0.88% . All the three L. monocytogenes isolates recovered in this study were found pathogenic based on haemolysis production (CAMP) and PI-PLC assay. All the isolates identified using conventional cultures & biochemical reactions were subjected for MALDI TOF MS analysis ; based on the score, isolates were confirmed with 100% agreements, this undercovered application of MALDI -TOF MS for the rapid and accurate detection of Listeria spp. Further all these L. monocytogenes isolates harbored virulence genes when tested using PCR for sic pathogenesis associated genes viz prsA, isp, iap, hlyA, actA, plcA. Antibiogram of isolates showed multiple drug resistance with higher resistance to amoxicillin/clavulanic acid, cefepime, oxacillin, sulphadiazine and trimethoprim (100%) followed by intermediately sensitive to cefotaxime and vancomycin. However isolates were found sensitive to azithromycin, cefalexin, ciprofloxacin, doxycycline, enrofloxacin, gentamicin, nalidixic acid, ofloxacin, streptomycin and tetracycline. Isolation of pathogenic & antimicrobial resistance L. monocytogenes from foods of animal origin. In the present study indicated potential public health risk access the food supply chain warranting prompt advising so as to protect the consumers health from this food safety hazard.
Subject: Veterinary Public Health
Theme: Occurrence, Characterization and Antibiogram Pattern of Pathogenic Listeria species
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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