Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810090098
Authors: Thakare, V. T.
Advisor: Waskar, V. S.
Title: Antimicrobial Resistance Pattern of Escherichiacoli from Foods, Water and Clinical Samples of Human and Animal Origin
Publisher: MAFSU, Nagpur
Language: en
Type: Thesis
Pages: 116
Agrotags: null
Keywords: Veterinary Public, Health, Antimicrobial Resistance, Escherichiacoli,Foods, Water, Clinical, Human, Animal Origin
Abstract: Escherichia coli from a total of 634 samples comprising of food of animal origin (334), environmental samples (180), human clinical samples (50) and animal clinical samples (50) procured from Udgir city and nearby villages/towns. Overall occurrence from food, environment, human and animal clinical samples was 9.62% (61/634) with productwise occurrence 10.47, 3.33, 14.00 and 18.57%, respectively indicating maximum occurrence in animal clinical samples and minimum in environmental samples. Matrixassisted laser desorption/ ionization time-of-flight mass spectrometry (MALDITOF MS) analysis characterized 28 presumptive E.coli isolates upto species level and 33 isolates were genus specific with probable species identification. In-vitro pathogenicity in the form of ability to produce haemolysin tested on congo red agar and sheep blood agar revealed that 26 isolates (42.62%) showed positive reaction. Maximum pathogenicity was exhibited by isolates recovered from animal origin food products (54.28%) followed by human clinical samples (42.85%), environmental samples (33.33%) and least from animal clinical samples (15.38%). PCR analysis targeting two virulenceassociated genes viz. temperature-sensitive haemagglutinin (tsh) and colicin V plasmid operon gene (cvi) revealed 26 (42.62%) isolates were positive for both virulence-associated genes, 4 (6.55%) were positive for only cvi and 2 (3.27%) isolates were positive for tsh only. Antibiogram pattern employing 15 different antibiotics revealed multiple drug resistance pattern and the maximum sensitivity was noted against azithromycin (80.32%), gentamicin (67.21%), ciprofloxacin (59.01%) and enrofloxacin (57.37%). Resistance was noted against amoxycillin-clavulanic acid (78.68%), lincomycin (77.09%), cefepime (63.93%), trimethoprim (60.65), vancomycin (59.01%), ofloxacin (57.37%), nalidixic acid (55.73%), cefotaxime (55.57%) and cefaclor and tetracycline (54.09% each). Highest resistance was exhibited against lincosamides followed by 2nd generation cephalosporin in animal origin food products; cephalosporins, glycopeptides, tetracyclines and lincosamidses in environmental samples as well as human clinical samples and β-lactams, cephalosporins, glycopeptides, tetracyclines and fluroquinolones in animal clinical cases.
Subject: Veterinary Public Health
Theme: Antimicrobial Resistance Pattern of Escherichiacoli from Foods, Water and Clinical Samples of Human and Animal Origin
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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