Please use this identifier to cite or link to this item:
Authors: Pandian, C
Selvan, ST
Parthiban, M
Karthickeyan, SMK
Title: A Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus)
Other Titles: International Journal of Current Microbiology and Applied Sciences
Publisher: Excellent Publishers
Language: en
Type: Article
Pages: 3073-3078
Volume: 6
Series/Report no.: ;9
Agrotags: Veterinary Science
Keywords: Protein bands, ostrich seminal plasma (OSP)
Abstract: The protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence. The results revealed that OSP had five major specific proteins bands namely, OSP-I (GATA Zinc finger domain containing protein 1), OSP-II (GATA Zinc finger domain containing protein 1), OSP- III (E3 ubiquitin protein ligase RNF 216, OSP-IV (Mitotic spindle assembly checkpoint protein MAD1) and OSPV (Dual specificity phosphatase DUPD1). The estimated molecular weight of OSP-I (Mr94.51 to 102.34 kDa), OSP-II (Mr75.19 to 93.07 kDa), OSP-III (Mr59.58 to 72.76 kDa), OSP-IV (Mr30.71 to 40.90 kDa) and OSP-V (Mr21.66 to 26.26 kDa). The results of our study provide basic knowledge of the protein composition of ostrich seminal plasma highlighting important physiological pathways which may play crucial roles in the sperm environment after ejaculation. This knowledge can be the basis to further develop procedures improving the reproduction of farmed ostrich.
Description: TNV_Ijcmas_2017_6(9)3073-3078
Issue Date: 2017-09
Appears in Collections:Articles (1.Journal)

Files in This Item:
File Description SizeFormat 
TNV_Ijcmas_2017_6(9)3073-3078.pdfTNV_Ijcmas_2017_6(9)3073-3078194.38 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.