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|Advisor:||Dr .P. KALYANI|
|Title:||MOLECULAR CHARACTERIZATION OF BACTERIAL PATHOGENS INVOLVED IN PNEUMONIA OF SHEEP AND GOAT|
|Abstract:||In the present study, a total of 112 bacterial isolates were recovered from 101 lung (64) and nasal discharge samples (48) of sheep and goat. Of 112 bacterial isolates, prevalence of bacterial sp was as follows; Staphylococcus spp., 55/112 (49.10%); and Klebsiella spp., 42/112 (32.75%) and Pseudomonas spp., 15/112 (14.1%) Bacterial isolates from different samples of respiratory infections in sheep and goat were evaluated using bacterial culture, and identification was confirmed by PCR and DNA sequencing. Universal primers targeting the bacterial 16S r RNA for bacteria were used for Pseudomonas aeruginosa and other sets of primers targeting spa and uge genes for Staphylococcus aureus and Klebsiella pneumoniae. The amplified PCR Created by Universal Document Converter products were subjected to DNA sequence analysis and these DNA sequences of the isolated bacteria were aligned with the DNA sequences of respective bacterial genes in the GenBank. Gene sequence studies demonstrated identification of the Staphylococcus aureus, Klebsiela pneumoniae, Pseudomonas aeruginosa from sheep and goat manifesting clinical signs of respiratory infection. Among 55 isolates of Staphylococcus sp. 14 (25.45%) isolates were positive for coagulase production and these isolates of Staphylococcus sp. were also tested for the detection of virulence marker spa gene by PCR. Of all 55 isolates investigated, the overall prevalence of spa genes was 72.72% and showing amplicon size of 920 bp. Klebsiella sp. recovered from samples were tested for the virulence marker uge gene by PCR. Out of 46 isolates 26 isolates (61.90%) were positive for uge gene showing amplicon size 534 bp. In the same way Pseudomonas sp. recovered from respiratory tract were tested for 16S rRNA and 9 out of 15 isolates (60%) were found positive. Upon sequencing these partial gene products, similarities with available sequences in GenBank, Nucleotide percentage varied from 37.4-100%, 36.6-100%, and 16.4-99.3% for spa, uge, and 16S rRNA genes respectively. From the current study findings it is concluded that a number of aerobic bacterial pathogens are associated directly or indirectly in respiratory infections of sheep and goats.|
|Appears in Collections:||Thesis|
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|2018 D586 B.Rajashekar VMC (Biotecnology)PG.pdf||8.59 MB||Adobe PDF|
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