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Authors: Meti, Netravati
Advisor: Samal, K.C
Publisher: Orissa Univesrity of Agriculture and Technology; Bhubaneswar
Language: en
Type: Thesis
Pages: 63
Agrotags: null
Keywords: Biochemical , Molecular, Characterization , Aromatic , Rice, Varieties , Odisha.
Abstract: "Rice, Oryza sativa (2n=24) belongs to the family Poaceae and subfamily Oryzoidea. It is the staple food for half of the world's population. It is grown under diverse cultural conditions as well as wide geographical range. Aromatic rice is a special group of rice having short to long-grained kernel. It is known for its aroma and taste, which is caused by the chemical compound 2-acetyl-I-pyrroline. A total of 48 aromatic genotypes were selected for exhaustive morphological, biochemical and molecular characterization. Morphological features like Plant height, days to flowering, panicle length, no. of fertile grains, 1000 grain weight and potential yield demonstrated a significant variation among the different genotypes under study. The plant height varied from 103.3 to 147.9cm, whereas the panicle length varied from 18.8 to 29.8 cm. There is a significant variation in days to flowering (91-107 days). Variation was also observed in number of fertile grains per panicle (62 to 195). The maximum potential yield was obtained in 'Mugajai' (29.42 q/ha) followed by 'Khosakani' (28.41 q/ha). The maximum starch content was observed in 'Ganjeikali'. Molecular markers were used to develop DNA fingerprints of 48 aromatic rice varieties. Eleven ISSRs primers representing di-and tri-nucleotide repeats generated distinct fingerprints of aromatic rice varieties. A total of 60 clear, distinct and reproducible amplified DNA bands were detected and out of which 51 bands (85%) are polymorphic. The number of DNA bands per marker ranged from 2 to 9, with an average 5.45 bands. The polymorphism information content (PIC) was the highest in ISSR primer AM-I (0.77) ranging from 0.30 (UBC-825) to 0.77 (AM-I). The average PIC was found to be 0.51. The genotype pair (, Dangerbasumati' and 'Gangaballi') showed the maximum similarity (0.93) among the 48 aromatic genotypes Cluster analysis using Jaccard's similarity index showed that all the genotypes under the present study could be grouped into two clusters, which correspond well with agromorphological characters. The maximum number of genotypes (43) was included in the Cluster I. Principal Component Analysis (PCA) indicated that of the total 48 genotypes of rice used in the present study, 'Kanakachamp', 'Kusumabhoga', 'Karpurakranti', 'Manasi- 1', 'Kalikati-2' was placed separately with respect to the rest of the genotypes in the coordinate axis. Similarly, ten SSR primers covering ten different chromosomes of rice genome were used for molecular analysis as well as diversity study. The number of bands produced varied with each primer, ranging from 1 (RM-44) to 5 (RM-20) with a mean of 2.20 per primer. Out of total 22 alleles generated, 20 alleles (90.90 %) were found to be polymorphic. The polymorphism information content (PIC) was 0.49 (RM-228) to 0.74 (RM-9, RM-218 and RM-30) with an average PIC value 0.58. At 53% similarity, forty eight rice genotypes were grouped into two clusters (I and II). There were 5 genotypes found in Cluster I and the rest 43 genotypes were grouped in Cluster II. Similar type of grouping was also observed in Principal Component Analysis (PCA). The genotype 'Ganjeikalli' alone formed a separate group. The genetic analysis based on ISSR and SSR is fairly strong to evaluate the genetic relationships of 48 aromatic rice genotypes. But the SSR marker was found to be superior to ISSR marker, as it is more informative as to genotypic discrimination. In essence, the present study is the foot step for molecular characterization of elite genotypes of aromatic rice varieties of Odisha that will help in study of marker validation for agronomically important characters, genome mapping and for recombination breeding programme for development of new cultivars."
Description: TH-3899
Subject: Agricultural Biotechnology
These Type: M.Sc
Issue Date: 2012
Appears in Collections:Thesis

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