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Authors: Mukesh Kumari
Advisor: AshokKumar
Title: Studies on microbial quality of marketed chicken meat
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: Staphylococcus, Salmonella, chicken meat, isolation, PCR, antibiotic
Abstract: In the present study, a total of 75 chicken meat samples were collected to evaluate their microbiological quality by analysis of total viable count and coliform count, detect presence of food borne pathogens viz., Staphylococcus and Salmonella and determine antimicrobial resistance pattern of Staphylococcus spp. isolates.Of all examined samples, 4% (2/50) raw chicken meat samples and 56% (14/25) ready-to-eat chicken meat samples had the counts within limit for total viable count. 7 out of 25 (28%) ready- to- eat chicken meat and all the raw chicken meat samples had total coliform count more than the permissible limits. A total of 43 (57.3%) isolates of Staphylococcus spp. were obtained, 33 from raw chicken meat samples and 10 from ready-to-eat chicken meat samples. Nineteen out of 43 isolates (44.1%) belonged to coagulase positive staphylococci and the rest 24 (55.8%) belonged to coagulase negative staphylococci group. Three (12%) out of 25 ready-to-eat and 16 (32%) out of 50 raw meat samples were positive for coagulase positive staphylococci. Sixteen (84.2%) out of 19 coagulase positive staphylococci were found to be Staphylococcus aureus. Six out of 50 raw chicken meat samples (12%) were positive for Salmonella whereas all the ready-to-eat chicken meat samples were found negative. Staphylococcus spp.isolates were found to be highly resistant against penicillin (93%), followed by cefoperazone (69.7%) and methicillin (55.81%). The lowest resistance (23.25%) was observed to chloramphenicol. Salmonella spp. isolates were found to be 100 % sensitive to amoxyclav, ciprofloxacin and ampicillin. The highest resistance (100%) was observed against enrofloxacin and moxifloxacin and ceftriaxone (83.33%). Out of six, 4 (66.67%) Salmonella isolates and 29/43 (67.44%) staphylococci were found to be multidrug resistant.Out of 19 coagulase positive staphylococci, 13 (68.42%), 8 (42.11%), 7 (36.84%) and 6 (31.58%) isolates carried mecA, gyrA, gyrB and grlA gene, respectively. The corresponding figures for coagulase negative staphylococci (n=24) are mecA=9 (37.5%), gyrA= 11 (45.83%), gyrB=2 (8.33%) and grlA=10 (41.67).Coagulase positive staphylococci and coagulase negative staphylococci differed significantly for carriage of mecA (p=0.044) and gyrB (p=0.022) genes.
Subject: Veterinary Public Health
Theme: Studies on microbial quality of marketed chicken meat
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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