Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810084267
Authors: SONI, NITIN
Advisor: Pandey, Anand Kumar
Title: Temporal and spatial expression profiling of MTNR1A gene in ovarian follicles of buffaloes
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: Murrah Buffalo, RNA, RT-qPCR, MTNR1A, RQ, Melatonin.
Abstract: The present study was undertaken to investigate the following two objectives: (i) Expression analysis of MTNR1A gene transcript in ovulatory size follicles of buffalo during summer and winter season by sensitive, quantitative and reliable real-time reverse transcription–polymerase chain reaction (RT-PCR) and (ii) To analyse and quantify the melatonin concentration in the follicular fluid of ovulatory size follicles of buffalo during summer and winter season. Buffalo ovaries were collected in both summer (May-June) and winter (December-January) season. All visible follicles were divided into three categories; Small (8-9.9 mm), Medium (10-11.9 mm) and Large (12-14 mm). Follicular fluid was aspirated individually from all categories follicles present on the surface of the ovaries. RNA was extracted from follicular walls in both seasons and, further was subjected to RT-qPCR for checking fold change in expression (RQ) of MTNR1A, ERα, ERβ and PR of each category of follicles. The expression of MTNR1A decreased with increasing size of the follicle and intrafollicular melatonin concentration. Expression of ERα and PR remained unaffected with the change in season and was found constant in all categories of follicles. Expression of ERβ was found higher in summer compared to winter season and small sized follicle of summer showed higher concentration of ERβ than winter season. The overall intrafollicular concentration of melatonin was found to be positively correlated with intrafollicular 17β estradiol and progesterone concentration. In conclusion, the decreased expression of MTNR1A with increasing size of the follicle and intrafollicular melatonin concentration, suggesting its probable role on oocyte maturation and ovulation process in Murrah buffalo.
Subject: Veterinary Gynaecology and Obstetrics
Theme: Temporal and spatial expression profiling of MTNR1A gene in ovarian follicles of buffaloes
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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