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Authors: Kumar, Devendra
Advisor: Kumar, Rajeev
Title: Molecular characterization of SS-III gene promoter with respect to Heat tolerance in Wheat
Publisher: Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur
Language: en
Type: Thesis
Pages: 66+iv
Agrotags: null
Keywords: Wheat, Heat tolerance, SS-III gene promoter, Molecular characterization
Abstract: Bread wheat (Triticum aestivum L.) is one of the most important staple food grains of the human race as it fulfils the significant proportion of dietary calories requirements of the world population. Starch is a major component of wheat flour, accounting for 65-70% of the dry weight of the wheat grain. There are 6 distinct classes of starch synthase: GBSS, SSI, SSII, SSIII, SSIV and SSV involved in the synthesis of starch. SS genes containing promoter of 2kb sequence were retrieved in wheat and other crop species using by the bioinformatics tools. The promoter sequence of the reference crop species or wheat was used as a query in the cereals database. 68 cis-acting regulatory elements and 118 total number of motif sequences were found across the species under study. A maximum number of motifs (51) were found in Amaranths spp. In wheat total, 27 motifs were found. ERE (Ethylene Responsive Element) was found in SS-III in wheat. G-box (cis-acting regulatory element involved in light responsiveness) and MBS (MYB binding site involved in drought-inducibility) were found in all the species of monocots and dicots. Four novel conserved motifs e.g. ARE (anaerobic induction); GC-motif (anoxic specific inducibility); TGA (auxin-responsive element); TGACG motif (MeJA responsiveness) were found in the SSIII gene promoter consensus sequence obtained through wet lab experiment but found absent in the retrieved sequences. In Phylogenetic analysis Maximum genetic similarities were observed for Phaseolus SS-I & Phaseolus SS-II and Arabidopsis SS-II & Arabidopsis SS-I respectively where-as maximum dissimilarities was observed between Phaseolus vulgaris L. SSII and Brachypodium SS-II. Wheat SS-I was found more closely related to its SS-III, SS-II was found closely related to Brachypodium SS-III, and SS-IV promoter was found closely related to SS-III of Zea mays. We identified a total of 72 SNPs were identified over a total region of 1496 bp. Out of 72, 24 SNPs are of transition types and 48 SNPs are of transversion types in the studied genotypes of wheat with different degree of sensitivity towards heat stress. In heat sensitive genotypes (PBW-343, SISD, HD-2967, and Sonara-64) there were 29, 12, 6, and 1 specific SNPs identified respectively. 7 and 17 specific SNPs were also identified in heat tolerant genotypes (KSG-1186) and (Ipecarabe) respectively. In heat tolerance genotypes (KSG-1186) and (Ipecarabe) identified more transversion types (A/T) SNP than transition types. Analysis of types of substitution among the identified SNPs revealed far more abundance of transversion than transition and the ratio of transition to transversion (Ts/Tv) as 0.5.
Subject: Agricultural Biotechnology
Theme: Molecular Characterization of SS-III gene promoter for Heat tolerance in Wheat.
These Type: M.Sc
Issue Date: 2018
Appears in Collections:Thesis

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