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Authors: Sharma, Himanshu
Advisor: Kapoor, Sanjay
Title: Studies on latency in equine herpesvirus-1 Infection among equines in India
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: EHV1, latency associated transcripts, equine herpesvirus latency
Abstract: Equine herpesvirus 1 (EHV1) is a common pathogen causing upper respiratory tract disease, abortion, neonatal death and equine herpesvirus myeloencephalopathy (EHM). EHV1 infection is characterized by acute disease followed by establishment of life-long latency. Although it is one of the most common pathogens of the horse causing massive outbreak of abortions, there is no information on prevalence of latent EHV1 infection in India. To achieve this, nested and real-time PCR assays were developed to detect EHV1 latency, targeting late structural gene (gB) (gB-nPCR, gB-qPCR) and latency associated transcripts (ORF63-nPCR, ORF64-nPCR, TaqMan ORF64-qPCR, SYBR ORF64-qPCR). In this study, attempt was made to establish a cell line in which virus could persist without producing its cytopathic effects. For this, human lymphoblastoid cells (LCs) were infected with EHV1 and observed for 120 days. Infectious extracellular and intracellular EHV1 was detected in LCs with mean titres of 104.67±0.09/million cells and 102.14±0.11/million cells, respectively. Virus in infected LCs was also demonstrated by immunofluorsence and OIE-nPCR. Latency associated transcripts (LAT) corresponding to ORF64 in EHV1-infected LCs were detected only from day 16 through day 120 post-infection. This is the first time report of expression of LAT in EHV1 persistently infected LCs. Further, we investigated an EHV1 associated natural abortion outbreak in 24 aborting mares for conversion to latent infection. Fifteen out of 24 mares became latently infected 6 months after outbreak. In order to detect the extent of latent EHV1 infection in equine population, we tested 119 mares of three different farms in Haryana and reported prevalence of latent infection in 42.01% animals in India. This is the first report describing prevalence of EHV1 latency in India which was was further confirmed by demonstration of LAT in 11 mares. All latently infected animals carried non-neurotropic strains of EHV1. This is the first report of detecting latent EHV1 infection in Indian horses supporting the reason for perpetuating EHV1 infection in India and uncommon occurrence of EHM.
Subject: Veterinary Microbiology
Theme: Studies on latency in equine herpesvirus-1 Infection among equines in India
These Type: Ph.D
Issue Date: 2018
Appears in Collections:Thesis

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