Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810082598
Authors: Asha
Advisor: Pawan Kumar
Title: Light microscopic studies on the oesophagus, crop, proventriculus and gizzard of broiler chickens of different age groups
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: Oesophagus, Crop, Proventriculus, Gizzard, Broiler Chickens
Abstract: The study was conducted on 30 broiler chickens of one week to 42 days age. The tissues were collected after post-mortem examination at 7, 14, 21, 28, 35 and 42 days of age. The tissues for histological and histochemical studies were collected from different sites of oesophagus, crop, proventriculus and gizzard along with their respective junctions. The oesophagus and crop were lined by stratified squamous keratinized to partly non-keratinized epithelium having mucous glands in lamina propria, whereas crop was devoid of these glands. Lymphoid aggregations were generally observed in between the clusters of glands in cervical oesophagus at 42 days age. Histochemical studies revealed existence of a high amount of different mucopolysaccharides and proteins in oesophageal glands. Only acidic mucopolysaccharides were observed as demonstrated by PAS-Alcian blue method. The PAS reaction was generally absent in crop except a very weak positive reaction was observed in the blood capillaries and blood vessels. The cervical oesophagus was externally covered by tunica adventitia whereas the thoracic oesophagus by serosa which was lined by mesothelium. At the junction of oesophagus and proventriculus, the glandular acini were infiltrated with lymphoid tissue and epithelium of these glands was modified into follicle associated epithelium (FAE) which was devoid of any PAS activity. The FAE at certain positions presented the cells which resembled the M-cells. Proventriculus and gizzard were lined by simple columnar type of epithelium. Proventriculus mucosa presented many folds and sulci. Submucosal glands formed the greater portion the organ. The height of the epithelium was more towards the plicae and comparatively reduced towards the bases of the sulci. The folds having concentric arrangement showed the presence of only acidic mucopolysaccharides in their cells with very less concentration of neutral mucopolysaccharides. The PAS reaction showing presence of glycogen was more toward plicae region. In PAS AB method, the columnar cells of plicae lining the luminal surface indicated predominance of neutral mucopolysaccharides with very less concentration of acidic mucopolysaccharides. Whereas, sulci in the deeper portion presented only the presence of acidic mucopolysaccharides and no neutral mucopolysaccharides were observed. Gizzard was characterized by an internal abrasion-resistance lining koilin, glycoprotein in nature, showed different affinity for PAS and thick muscular layer. The lamina muscularis mucosae was absent. The mucosal epithelium lining of the surface and gastric pits of glands indicated the presence of both neutral and acidic mucopolysaccharides. The number of cell layers in different strata, concentration of glands and thickness of the muscle layers increased with advancement of age.
Subject: Veterinary Anatomy
Theme: Light microscopic studies on the oesophagus, crop, proventriculus and gizzard of broiler chickens of different age groups
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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