Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810082595
Authors: Tiwari, Sakshi
Advisor: Jakhar, K. K.
Title: Pathobiological and immunological studies on deltamethrin toxicity and its interaction withe escherichia coli infection in broiler chicken
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: Chicken, Deltamethrin toxicity, Immune response, Pathology, E. coli etc
Abstract: The present study was undertaken in 270 broiler chickens to access the effects of deltamethrin toxicity and its interaction with colibacillosis infection. Maximal tolerated dose (MTD) of deltamethrin was calculated 43.75 mg/kg using 40 birds. Two hundred and thirty broiler chicks at the age of seven days were divided randomly into three groups viz., group A, B and C containing 70, 80 and 80 chicks, respectively. Birds of group B was given 1/5th of MTD of deltamethrin (8.75 mg/kg) orally through drops and birds of group C was given 1/10th of MTD of deltamethrin (4.375 mg/kg) throughout the experiment. The group A was kept as control i.e. no deltamethrin was given. At the age of 14 days, the birds of groups B and C were divided into two subgroups (group B into B1 and B2, group C into C1 and C2) of 40 birds each. Similarly group A was also divided into two subgroups (A1 and A2) of 30 and 40 birds, respectively. Thereafter, each bird of subgroup A2, B2 and C2 was injected intraperitoneally 107 CFU E. coli in 1 ml normal saline solution. Total duration of experiment was 42 days. Clinical signs of colibacillosis infection such as dullness, depression, drooping of the head and neck, closing of eyes and ruffled feathers were more profound and severe in chicks of group B2 followed by C2 and A2. Deltamethrin intoxicated groups B1 and C1 showed salivation, whitish diarrhoea, listlessness and depression after 10 and 15 days post exposure and continued throughout the experiment. Mortality in group B2 was higher as compared to groups C2 and A2. Mean body weight in groups A2, B2 and C2 significantly decreased from third day post infection with maximum reduction in group B2. Deltamethrin intoxicated groups B1 and C1 showed non-significant reduction in body weight as compared to control on 28 DPDT. Serum biochemical studies revealed significant reduction in total proteins and albumin content in all infected groups and deltamethrin intoxicated groups as compared to control. Serum AST, ALT, LDH, ALP, BUN and serum creatinine values showed significant increase in all infected groups and deltamethrin intoxicated groups as compared to control with marked increase in groups B2 followed by C2 and A2. Oxidative stress parameters such as SOD, catalase and GSH showed significant reduction, whereas LPO and protein carbonyl content showed significant increase in infected groups and deltamethrin intoxicated groups. Immunopathological studies revelaed no significant difference in mean reciprocal log10 antibody titer in all three infected groups. Mean increase in skin thickness due to DTH was maximum in control group A1 followed by C1, B1, A2, C2 and minimum in group B2 with non significant variations. Pathological studies revelaed characteristic gross lesions of colibacillosis in infected groups like fibrinous pericarditis, perihepatitis and enteritis. Chicks of deltamethrin toxicity alone showed anaemic carcass, mottled enlarged liver, haemorrhagic enteritis, and congested liver, lungs, heart, intestine, spleen, thymus, pancreas and brain with more severe lesions were appreciated in group B1 than group C1. Histopathological lesions observed due to colibacillosis were congestion, fibrinous perihepatitis characterised by large amount of fibrin and infiltration of leucocytes, pericarditis, serofibrinous pneumonia with mononuclear cells infiltration in lung parenchyma, focal interstitial nephritis, haemorrhagic enteritis, pancreatitis, congestion in proventriculus, depletion of lymphocytes in lymphoid organs and congestion in brain along with meningitis and encephalitis. Deltamethrin alone intoxicated groups revealed mild congestion in liver with focal area of necrosis, pulmonary oedema, pericarditis, focal interstitial nephritis, depletion of lymphocytes in lymphoid organs, congestion in pancreas, proventriculus and brain. Based upon present studies, it is concluded that deltamethrin intoxication enhanced the severity of the colibacillosis infection and impaired cell mediated immune response.
Subject: Veterinary Pathology
Theme: Pathobiological and immunological studies on deltamethrin toxicity and its interaction withe escherichia coli infection in broiler chicken
These Type: Ph.D
Issue Date: 2018
Appears in Collections:Thesis

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