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Authors: Puneet Ranjan
Advisor: Vinay Ganeshrao, Joshi
Title: Serodiagnosis and molecular characterization of newcastle disease virus and infectious bronchitis virus in high altitude region of leh-ladakh
Publisher: LUVAS
Language: en
Type: Thesis
Agrotags: null
Keywords: Newcastle disease virus (NDV), Infectious Bronchitis Virus (IBV)
Abstract: Newcastle disease (ND) and Infectious bronchitis are two most important viral diseases of poultry caused by avian Paramyxovirus and Coronavirus respectively. The morbidity and mortality of a flock varies as per the strain involved, which varies from Per-acute disease with almost 100% mortality to Sub-clinical disease with no lesions. The samples which included serum, tissue and cloacal swabs, were collected from various areas of Leh-Ladakh. Tissue samples were checked for presence of NDV and IBV infection by using appropriate primer and comparing it with the result of positive control. A total of15 isolates were prepared by inoculating it in SPF eggs with minimum of 3 passages. The tissue samples of Leh-Ladakh which were found positive for NDV were showing 94-98% identity to that of Genotype VII (Class II) viruses isolated from Pakistan. Only clocal swab sample which was positive for IBV was showing identity to that of mass strain. The tissue samples of Haryana which were positive for NDV were found to be showing identity to that of Genotype II (Class II) viruses, while the tissue samples which were positive for IBV were showing identity to that of H-120 strain. Serum Samples were checked for both NDV and IBV, and it was reported that a significant amount of samples were positive for NDV or IBV or both. Therefore, it can be concluded that both NDVs and IBVs are circulating in Leh-Ladakh. The vaccination programme for the control of thesediseases can provide protection against the prevalent genotypes in the environment in this region. Further, regular monitoring and surveillance program is warranted in this high altitude region to make them disease free zone. Owing to the fact that pigeons and some migratory are very common in areas of Leh-Ladakh, a further study regarding prevalence of NDV and IBV in wild birds is needed to ascertain the origin and spread of these viruses MAJOR ADVISOR (SIGNATURE OF DEGREE HOLDER) HEAD OF
Subject: Animal Biotechnology
Theme: Serodiagnosis and molecular characterization of newcastle disease virus and infectious bronchitis virus in high altitude region of leh-ladakh
These Type: M.V.Sc.
Issue Date: 2018
Appears in Collections:Thesis

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