Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810082021
Authors: Kumar, Sanjeev
Advisor: Sahni, Sangita
Title: Integrated disease management of Chickpea Fusarium wilt
Publisher: Dr. Rajendra Prasad Central Agricultural University, Pusa, Samastipur
Language: en
Type: Thesis
Pages: 55+xv
Agrotags: null
Keywords: Chickpea, Fusarium wilt, Disease control
Abstract: Chickpea (Cicer arietinum) is one of the world’s major legume crops and suffers substantial damage from wilt disease incited by Fusarium oxysporum f. sp. ciceri (Padwick) with yield loss over 60 per cent. Itis an important soil borne plant pathogen and is difficult to manage by application of chemical. Moreover, the chemical control is costly and leads to residual effect. In the present study, an integrated approach using host plant resistance and integration of three components i.e., cultural (vermicompost amendment), chemical (ZnSO4 and Salicylic acid) and biological (seed bacterization with Pseudomonas spp.) were contemplatedto manage this disease more effectively. A total 55 chickpea genotypes were screened against Fusarium wilt, only one genotype was found to be resistant, however, twelve were found to be moderately resistance (10.1-20 per cent), nineteen were moderately susceptible (20.1-30 per cent), and nineteen genotypes were showed susceptible reaction for wilt disease and recorded more than 30 % wilt incidence and four genotypes showed highly susceptible reaction exhibited more than 50% wilt incidence. The pathogen associated with wilt of chickpea was isolated from the infected plant samples and identified as Fusarium oxysporum f. sp. ciceris based on standard mycological keys. A total of nine pseudomonad isolates were screened for their antagonistic activities against F. oxysporum f.sp. ciceri. All nine isolates exhibited the ammonification property, produced HCN (Cyanide) and IAA (Indole acetic acid) and positive for phosphorus solubilization, except PGPR-Pl, this found to be negative for HCN production. Among the nine isolates, pseudomonad isolates PGPR-WS were best in exhibiting multiple PGPR traits like ammonification, HCN production, IAA production (26.08 mgl-1), and phosphate solubilization (306.51 mgl-1) as well as best in antagonistic activity against F. oxysporum f.sp. ciceri, showed 75.00 % inhibition of growth of mycelia over control and caused total lysis of mycelia in advanced stages of antagonism. Thus screening and identification of novel bioagent PGPR-WS with multifarious activities vividly reflects its potential to suppress F. oxysporum f.sp. ciceriand suggest usefulness of this super bioinoculant as component of IDM of F. oxysporum f.sp. ciceri Among the three control methods, viz., vermicompost amendment, foliar spray with non-conventional chemicals and seed bacterization with PGPR-WS, when used singly, seed bacterization with PGPR-WS was found to be the most effective one among all against F. oxysporum f.sp. ciceri. It is also evident that the biocontrol efficiency against wilt pathogen could be improved by proper combination of vermicompost amendment as well as non-conventional chemicals, as they possess different modes of action against F. oxysporum f.sp. ciceri. Our findings indicated that the high antagonistic activity of PGPR-WS permitted the combination of chemical (non-conventional chemicals like Salicylic acid andZnSO4) and cultural (vermicompost amendment) measures, leading to improved control of the wilt pathogen of chickpea. The combination of all the three control methods was more effective than the lone treatment of the non-conventional chemicals, PGPR-WS, and vermicompost amendment. However, combined application of 15 % vermicompost and seed bacterization with PGPR-WS along with pre-inoculation foliar spray of salicylic acid showed complete protection against F. oxysporum f.sp. ciceri. Among the chemicals tested, Salicylic acid (40 ppm) was usually the most effective one in reducing wilt incidence of chickpea, compared to ZnSO4 (50ppm).
Subject: Plant Pathology
Theme: Fusarium wilt disease of chickpea
These Type: M.Sc
Issue Date: 2018
Appears in Collections:Thesis

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