Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810071907
Authors: SHIVA PRASAD, Ch.
Advisor: Ramesh Gupta, B(MAJOR)
Venkatramaiah, A
Chatterjee, R.N
Title: PHENOTYPIC AND MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS
Publisher: SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Language: en
Type: Thesis
Pages: 145
Agrotags: null
Keywords: DAHLEM RED LAYERS;PHENOTYPIC;MOLECULAR GENETIC
Abstract: ABSTRACT: Thc investigation was made on full sib, half sib and non-inbred groups of Dahlem Red birds maintained at the Project Directorate on Poultry. Hyderabad. to characterize then phenotypically and by RAPD markers. The phenotypic characterization war undertaken on a total of 328 Dahlem Red birds belonging to full sib mated (45). half sib mated (64) and non-inbred groups (219). The overall icast squares means of body weight at 4. 8. 20, 40. weeks of age. ASM. EP 40. EW 32 and EW 40 were 146.88. 374.28. 1150.70. 1678.57 g. 181.02 days, 71.06, 52.81g and 56.25 g. rcspectivoly. The mean performance of non-inbred group was in general. superior to inbred groups in dl the traits studied. The estimates of heritability obtained by half sib mehod for the full sib. half sib and non-inbred groups were 0.02. 0.61 and 0.49. respectively for BW 4 and 0.19, 0.20 and 0.53. rrspectrvely for BW 40. For egg weights. the highest heritabilities were obtained in half sib group and the lowest in non-inbred group. Majority of the genetic comlations of BW 20 md BW 40 wih egg weight were positive. The genetic comlii~ionosf ASM with EP 40 and egg weights were negative. The EP 40 was also found to be correlated negatively with EW 32 and EW 40. Majonfy of tht phcnotyp~cc omlations between the body weight and egg we~ghtw ere positive, while che comlations of ASM with EP 40 were negative. The phenotyp~c conrlarions of ASM with EW 32 and EW 40 mgcd from -0.18 to 0.17. where&\ the comlations between EP 40 and egg weights were negative in half sib and non-inbred grvups * - - - -- Fn an --A ,,, ...-:nt,, 0 13 The phenotypic comlations estimated by full sib method among the body weights were in general positive and ranged from -0.11 t 0.34 in full sib group. The BW 4 and BW 8 were comlated negatively with ASM. while BW 20 and BW 40 were also correlatd negatively with EP 40. The phenotypic correlations of BW 4. BW 8, and BW 40 with egg weights were found to be positive. The ASM was comlated negatively with EP 40 (-0.58). whereas, the correlations of EP 40 with EW 32 and EW 40 were positive. The phenotypic comlations among the body weights in half sib group ranged from -0.06 to 0.46; The phenotypic correlations between body weights and ASM varied from -0.19 to 0.30, whereas the comlations of body weights with EP 40 ranged from -0.10 to 0.34. The phenotypic correlations between body weights and egg weights at various ages were all positive. For molecular genetic characterization, a total of 48 Dahlem Red birds. ( 16 birds each of the group) with equal representation of sexes, were utilized. A total of 100 Random Amplified Polymorphic DNA (RAPD) primers were tested, of which, 21 primers generated appreciable polymorphism. A total of 341 RAPD bands were produced, out of which 204 wen found to be polymorphic. The overall mean number of bands produced by the primer varied from 12 to 23. The size of amplified bands varied from 100 bp to 3409 bp. The analysis of the fingerprint patterns of individual DNA samples revealed that the full sib group was unique by the pre.xnce of one 1866 bp fingerprint when amplified by the primers PDP157F and 496 bp and 380 bp fingerprints only when amplified by the primer PDP176F. The half sib group wa\ unique by the presence of 1788 bp fingerprint when amplified by the primer PDPSOF. The non-inbred group could be distinguished by the prcsence of a unique 42 1 hp band bawd upon PCR amplification with primer PDP54F and PDP73F. The overall genetic sirn~lwitiesh a.sed on band sharing within the full sib, half sib and non-lnhred group were 87.90. 87.85 and 83.94 per cent. respectively, whereas the genetic similarities between full sib and half sib, half sib and non-inbred and full sib and non-inbred groups were 90.18. 88.51 and 88.07 percent, respectively. The overall genetic similarities based on band frequency within full sib, half sib and non-inbred groups were 85.25. 85.09 and 8 1.14 per cent, respectively, whereas those between full sib and half sib, half sib and non-inbred and full sib and non-inbred groups were 79.53, 74.34 and 72.78 per cent, respectively. The genetic distance between full sib and non-inbred groups was found to be the maximum (0.3 176). while the minimum genetic distance (0.2290) was observed between full sib and half sib groups. The RAPD markers in the present study were able to detect the polymorph~sm between the three groups of Dahlem Red i.e., full sib mated, half sib mated and non-inbred and could establish the genetic relationships
Description: THESES
Subject: Animal Genetics and Breeding
Theme: PHENOTYPIC AND MOLECULAR GENETIC CHARACTERIZATION OF DAHLEM RED LAYERS
These Type: M.V.Sc.
Issue Date: 2006-03
Appears in Collections:Thesis

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