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|Advisor:||Dr. K. VIJAYA LAKSHMI|
|Title:||SYNERGISTIC EFFECT OF ORGANIC ACID (Lauric acid) AND PROBIOTIC (Bacillus subtilis PB6) ON THE PERFORMANCE OF COMMERCIAL BROILER CHICKEN|
|Abstract:||This study was conducted to evaluate the synergistic effect of organic acid (lauric acid) and probiotic (Bacillus subtilis PB6) on growth performance, carcass parameters, immune response, nutrient retention (DM, CP and Energy), total bacterial count, gut pH and cost economicsin commercial broiler chickens. Two hundred and fifty (250), day old male broiler chicks were randomly allotted to five dietary treatments with ten replicates of five birds each and reared in battery brooder up to 42 days of age. At the end of experiment, ten birds from each treatment were sacrificed to evaluate the effect of organic acid (OA) and probiotic (PB) on carcass parameters. A metabolic trial of three days duration from 43rd to 45th day was conducted by housing one bird per replicate in a metabolic cage. Excreta of the birds were collected consequently for three days and oven dried to know the percent dry matter for the estimation of protein and energy digestibility. Blood samples were collected from one bird per replicate on 36th day of the experiment for studying humoral immune response (NDV titers) andfor cell mediated immune response, 100 μg of PHA-P suspended in 0.1 ml of phosphate buffer saline was injected into foot web on 40th day and skin thickness was measured on 41st day of the experiment.Broilers were fed on corn-soyabean based broiler pre-starter (0-14d), starter (15-28d) and finisher (29-42d) diets to meet the requirements. Five experimental diets were prepared in which, T1 was control diet without 16 any growth promoter, T2 with 0.05% antibiotic (bacitracin methylene disalicylate), T3 with 0.05% organic acid (lauric acid), T4 with 0.1% probiotic (Bacillus subtilis PB6) and T5 with 0.05% organic acid + 0.1% probiotic (lauric acid + Bacillus subtilis PB6). Results showed that cumulative body weight gain and feed consumption was significantly (P<0.05) higher in birds supplemented with OA and PB in combination and alone compared to the birds fed with AB and control diet at 42d of age. OA+PB supplementation has increased body weight gain by 19.9% compared to those birds fed control diet. Cumulative feed conversion ratio was significantly (P<0.05) better with OA+PB (1.77) supplementation followed by OA (1.79) supplementation compared to the birds fed with AB and control diets (1.87 and 1.94, respectively). Mortality was relatively higher in control and AB fed birds compared to the birds fed with OA and PB diets. Supplementation of OA and PB in combination or alone had no significant (P>0.05) effect on carcass parameters except for percent breast yield and thymus weights which were significantly (P<0.05) higher in birds supplemented with OA and PB compared to control birds. OA+PB inclusion in diets had significantly increased breast yield by 18.4% over control group. Humoral immune response (NDV titers) was significantly (P<0.05) higher in birds fed with OA+PB followed by PB compared to the birds fed with control and ABand no significant effect was observed on cutaneous basophilic hypersensitivity response by different treatments. The total bacterial count was significantly (P<0.05) reduced due to the inclusion of OA followed by OA+PB in diets compared to the birds fed with control, AB and PB. Supplementation of OA and PB in diets significantly (P<0.05) reduced the pH in proventriculus and gizzard compared to the birds fed with AB and control diets. In duodenum, the pH was reduced by OA supplementation compared to other treatments and no significant effect was observed in the ileal pH. Inclusion of OA and PB in the diets of broilers had no significant effect on dry matter and crude protein retention but energy retention was significantly (P<0.05) higher with OA, PB and AB fed birds compared to control. OA in combination with PB or alone in broiler diets was economically better (P<0.05) compared to AB and control birds. Considering the above results, it could be concluded that supplementation of OA (lauric acid) and PB (Bacillus subtilis PB6) in combination had significantly improved the overall performance of broilers in terms of body weight gain (19.9%), feed efficiency and also breast meat yield (18.4%) with better humoral immunity compared to other treatments showing their synergistic effect|
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