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JAGADEESHA, R. C.
SHANKARAPPA, K. S
|Title:||MARKER ASSISTED BREEDING AND GENE ACTION STUDIES IN FRENCH BEAN (Phaseolus vulgaris L.)|
|Publisher:||K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)|
|Abstract:||French bean (Phaseolus vulgaris L.) is an important legume vegetable. It is grown for its tender pods either for fresh consumption or for processing as canned and freez dried products. Stringlessness is a temperature dependent trait. Beans that are stringless at cool temperatures form strings at high temperatures due to the modifier genes. Hence, this definitive molecular marker study was undertaken to mitigate the occurrence of stringed and stringless individuals. Ring Beans, IC 280007 and Gokak Local, EC 024948 genotypes were used as stringless and stringed parents respectively. F2 populations were developed for identification of linked markers. Eight polymorphic markers (B4, B6, B14, B16, D16, J13, L9 and I10) were identified for stringless and stringed parents from a group of 144 SRAP primers. Primer L9 (Me12, Em9) produced hybrid specific band of 500bp and B4 (Me2, Em4) produced hybrid specific band of 300 bp size which might be linked with our trait of interest in the population Gokak Local x Ring Beans and EC 024948 x IC 280007 respectively. RAPD and ISSR markers failed to produce any amplification. Since French bean is being a highly self pollinated crop, it will inherit all those genes which are present in the F1 individual. Hence having gene/s controlling stringlessness in F2 individuals is quite possible. In the cross Gokak Local x Ring Beans mode of gene action study was also estimated through five parameter-five generation mean analysis. Predominance of dominance gene effects were found for most of the characters where as epistatic component was expressed for number of seeds per pod. Among two segregating population, most of the characters had superior segregants in F2 population. Recurrent selections like, diallel selective mating or biparental mating in early segregating generation and selection followed by hybridization might prove to be alternative approaches to improve the traits.|
|Theme:||MARKER ASSISTED BREEDING AND GENE ACTION STUDIES IN FRENCH BEAN (Phaseolus vulgaris L.)|
|Research Problem:||MARKER ASSISTED BREEDING AND GENE ACTION STUDIES IN FRENCH BEAN (Phaseolus vulgaris L.)|
|Appears in Collections:||Theses|
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