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|Title:||MICROPROPAGATION AND MUTATION STUDIES IN POMEGRANATE (Punica granatum L.)|
|Publisher:||K.R.C. COLLEGE OF HORTICULTURE, ARABHAVI (UNIVERSITY OF HORTICULTURAL SCIENCES, BAGALKOT)|
|Abstract:||A study was undertaken to standardize in vitro plant regeneration through direct and indirect regeneration of pomegranate cv. Kesar and Ganesh and mutation studies carried out by using chemical (EMS 0%, 0.1%, 0.3%, 0.5%, 0.7%, 0.9% of 1, 2, 3 and 3.5 hr) and physical (gamma rays 0, 10, 15, 20, 25 Gy) mutagens for screening against bacterial blight of pomegranate. For direct regeneration was attempted using different explants viz., shoot tip nodal explant, cotyledon and hypocotyls segment. These were cultured on initiation media full strength of MS and WP media with 35 mg/l, citric acid for establishment of culture. One month after initiation, culture with transferred to multiplication media with BAP 1 mg/l and NAA 0.5 mg/l full strength MS and WP media for shoot multiplication. Cotyledon (69.02%) explant showed good response for direct regeneration among different explant among cv. Kesar (51.58%) it was good, MS medium (53.74%) supportted better growth. ½ strength of MS and WP media with different concentrations of auxin with (BAP 0.5, 1.0 mg and NAA 1 mg) were used. Rooting efficiency was good with NAA 0.5 mg in WP media (57.10%) background (highest rooting percentage, number of roots and more root length). To get true to type sapplings nodal explants may be preferred. Nodal segment (1.83 g) produced good quality of calli in MS media (1.11 g) with 4 mg/l BAP. Among the cultivars Kesar (1.14 g) gave good response for induction of calli compared to Ganesh. In vitro shoots are transferred to multiplication media after one month of sub-culture. Pomegranate seeds and cotyledon explant treated with gamma rays and EMS respectively then screened against bacterial blight of pomegranate isolated toxin added to initiation media but EMS treated cotyledon explant did not survive. The treatment 10 and 25 Gy showed less and equal infection to bacterial blight infection when artificially screened with X. auxonopodis pv. punicae.|
|Theme:||MICROPROPAGATION AND MUTATION STUDIES IN POMEGRANATE (Punica granatum L.)|
|Research Problem:||MICROPROPAGATION AND MUTATION STUDIES IN POMEGRANATE (Punica granatum L.)|
|Appears in Collections:||Theses|
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