Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810056507
Authors: DEEPAK KOUJALAGI
Advisor: Dr. S. A. DESAI
Title: MARKER ASSISTED BREEDING FOR LEAF RUST RESISTANCE IN BREAD WHEAT (Triticum aestivium L.) FOR RAINFED ECOSYSTEM
Publisher: University of Agricultural Sciences Dharwad
Language: en
Type: Thesis
Pages: 127
Agrotags: null
Keywords: MARKER ASSISTED BREEDING FOR LEAF RUST RESISTANCE IN BREAD WHEAT (Triticum aestivium L.) FOR RAINFED ECOSYSTEM
Abstract: The present investigation was primarily undertaken to genetically transfer two wheat leaf rust resistance genes, Lr24 and Lr28 into an elite, susceptible bread wheat cultivar MP3299 and to evaluate segregating population of MP3299 x NIL PBW343 for leaf rust resistance under artificial leaf rust conditions and to confirm the plants for presence of Lr24 and Lr28 genes through flanking molecular markers. The SCAR markers SCS73719 and SCS421570 linked to Lr24 and Lr28 respectively, along with SSR marker wmc313 for Lr28 were utilized for MAS of individual plants possessing the targeted genes. Genotyping of F3, F4 and F5 plants with SCAR markers revealed that 71, 122 and 10 plants respectively exhibited the presence of both the resistance genes. In F5 generation, six plants identified to be homozygous for Lr28 gene with the help of SSR marker Wmc313 linked to Lr28 gene. Evaluation of individual leaf rust resistance genes like Lr24 and Lr28 as well as both Lr24 and Lr28 in F4 and F5 populations indicated the superior performance of plants carrying both Lr24 and Lr28 as compared to plants with individual leaf rust resistance genes Lr24, followed by Lr28. Thus identified lines are potential resistance sources for future breeding programmes aimed towards identification of superior recombinants and are promising genotype for rain fed ecosystem. High frequency of transgressive segregants carrying both Lr24 and Lr28 genes in F4 and F5 generation was recorded for productive tillers per plant, spikelets per spike and thousand grain weights. Moderate frequencies of transgressive segregants have been observed for plant height and spike length. High frequencies for traits like productive tillers per plant, spikelets per spike and thousand grain weights, indicate that population is having good potential for selection of plants for these traits.
Subject: Genetics and Plant Breeding
Theme: MARKER ASSISTED BREEDING FOR LEAF RUST RESISTANCE IN BREAD WHEAT (Triticum aestivium L.) FOR RAINFED ECOSYSTEM
These Type: M.Sc
Issue Date: 2015-06
Appears in Collections:Thesis

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