Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810055885
Authors: PRASHANT SHETTI
Advisor: SOKKA REDDY, S.
Title: CONVERSION OF PARENTAL LINES OF DHM119 MAIZE HYBRID INTO QPM VERSION EMPLOYING MARKER ASSISTED SELECTION
Publisher: Professor Jayashankar Telangana State Agricultural University
Language: en
Type: Thesis
Agrotags: null
Keywords: CONVERSION PARENTAL LINES DHM119 MAIZE HYBRID QPM VERSION EMPLOYING MARKER ASSISTED SELECTION
Abstract: Enrichment of nutritional quality in maize has become essential in the malnourished population of the world. Several breeding efforts were initiated earlier to develop QPM hybrids and recently marker assisted selection is the most feasible way of developing QPM hybrids in short time. The present investigation deals with conversion of elite normal maize inbred lines BML2 and BML15 (parental lines of DHM119 maize hybrid) into QPM lines using marker assisted selection. Breeding efforts have led to the successful development of QPM cultivars and to improve the efficiency of QPM breeding, the utility of three simple sequence repeats (SSR) markers viz. umc1066, phi057 and phi112 were used in selection and introgression of the opaqaue2 gene. Polymorphism was detected between recipient parents (BML2 and BML15) and donor parent (CML142) with phi057 SSR marker. Foreground selection was carried out in each generation using opaque2 specific marker phi057. Recombinant selection was done using flanking markers to minimize the linkage drag and background selection was carried out in BC1F1 and BC2F1 generations to recover the recurrent parent genome (RPG) using genomic polymorphic SSR markers distributed across the genome. In BC2F1 the recovery of recurrent parent ranged between 90 to 93% and the plants with highest recovery were selfed to generate advanced generations (BC2F2 and BC2F3). Kernels were screened for endosperm hardness using light box and kernels showing less than 25% opacity were selected and further forwarded. Rigorous phenotyping was done for various plant characters and tryptophan was estimated using colorimetric method. Tryptophan content varied from 0.89% to 0.96% in converted lines of BML2 and 0.89% to 0.98% in converted lines of BML15. These normal looking converted inbreds named as CBML2 and CBML15 can be further used for reconstitution of the DHM119 maize hybrid. Key words: QPM- Quality protein maize, opqaue2, tryptophan, recurrent parent genome
Subject: Biotechnology
Theme: Biotechnology
These Type: Ph.D.
Issue Date: 201
Appears in Collections:Theses

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