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Authors: Gaikwad, Nilesh Dattatraya
Advisor: Shende, S. S.
Title: Synthesis and evaluation of antimicrobial potential of copper Nanoparticles
Publisher: Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Language: en
Type: Thesis
Pages: 72
Agrotags: null
Keywords: Drip, Irrigation, Fertigation, Turmeric, Sulphuric acid
Abstract: Development of green nanotechnology is generating interest of researchers toward ecofriendy biosynthesis of nanoparticles. In this study, biosynthesis ol stable copper nanoparticles was acheived using Ocimum sanctum leaf extract, These biosynthesized copper nanoparticles were characterized by using UV-Vis spectroscopy showed absorption of copper nanoparticles at 345nm. FTIR spectrum of copper nanoparticles suggestes that copper nanoparticles are surrounded by different organic molecules such as terpenoids, alcohols, ketones, esters, aldehydes and carboxylic acid. TEM analysis showed that the copper nanoparticles were rod, cylindrical and elliptical. The average particle size of copper nanoparticles was 25 nm with total concentration 300.29 particles/frame, 13.53 particles/ml and -14.9 mV zela potential value. The maximum activity of copper nanoparticles was found bacterial pathogen against Xanthomonas axonopodis pv. punicae (17.25±0.95) while the minimum activity was found against Razoctonia solani (10±0.81) with 0.01 mg/ml and 0.03mg/ml MIC respectively. The maximum activity of copper nanoparticles was found against fungal pathogen Alternaria carthami (18.5±1.7) while the minimum activity was found against Rhizopus stolonifer (10.5±0.5). The MIC of the copper nanoparticles against fungus was found to be 0.06 mg/ml for Alternaria carthami. Aspergillus niger, Fusarium oxysporum f.sp udum and 0.03 mg/ml for Colletotrichum gloeosporioides, Colletotrichum lindemuthianum, and Drechslera sorghicola Fusarium oxysporum f.sp. carthami, Fusarium oxysporum f.sp. ciceri, Macrophomina phaseolina, Rhizoctonia bataticola and Rhizopus stolonifer. Thus, this method can be used for rapid and ecofriendly biosynthesis of copper nanoparticles and protection against phytopathogens.
Subject: Agricultural Biotechnology
Theme: Agriculture
These Type: M.Sc
Issue Date: 2015-05-30
Appears in Collections:Thesis

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