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Authors: Aghav, Dinesh Damodhar
Advisor: Kamble, K. R.
Title: Characterization of sunflower (Helianthus annuus L.) genotypes differing for reaction to downy mildew (Plasmopara halstedii) using molecular markers
Publisher: Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani
Language: en
Type: Thesis
Pages: 91
Agrotags: null
Keywords: Lactation, Murrah buffalo, Calving
Abstract: The aim of this work was the application of previously developed PCR markers for the evaluation of sunflower genotypes differing for downy mildew. Sunflower genotypes were investigated. Genomic DNA was extracted from the first pair of leaves, and its polymorphism was investigated by 10 RAPD, and 10 SSR published markers for disease resistance. 10 RAPD primers produced a total of 1162 amplified products. Among these 802 were polymorphic with an average of 69.01 per cent polymorphism. Out of the ten primers used for amplification. Three primers (OPB-12, OPB-15 and OPB-07) showed 100 percent polymorphism. The primer UBC-324 gave the lowest polymorphism (38.46%). The number of bands ranged with an average 21.97 bands per primer. As in 10 SSR primers produced a total of 217 amplified products. Among these 77 were polymorphic with an average of 35.48% polymorphism out of the ten primers used for amplification. One primer (ORS-705) showed 100 per cent polymorphism. The number of bands ranged with an average of 21.07 bands per primer. In order to select genotypes for making crosses for use in further investigation, the obtained results were also used for the calculation of genetic distances between genotypes (similarity coefficient values) and the construction of a dendrogram (UPGMA) method and specific primers have been used for the investigation of the resistant, susceptible and tolerant sunflower genotypes in this present study.
Subject: Agricultural Biotechnology
Theme: Agriculture
These Type: M.Sc
Issue Date: 2015-09-28
Appears in Collections:Thesis

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