Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810044086
Authors: BALASAHEB BIRADAR
Advisor: Dr. C. R. PATIL
Title: METAGENOMIC ANALYSIS OF PANCHAGAVYA
Publisher: University of Agricultural Sciences Dharwad
Language: en
Type: Thesis
Pages: 119
Agrotags: null
Keywords: METAGENOMIC ANALYSIS OF PANCHAGAVYA
Abstract: Panchagavya is the blend of five ingredients obtained from cow. The present research focused mainly on studying the microbial succession at different stages of panchagavya making use of culture independent approach. The DNA isolation protocols for panchagavya samples drawn at different intervals, individual ingredients, phylloplane of soybean sprayed with panchagavya along with their respective control were standardized. Specific primers targeting variable region of 16S rRNA were used in PCR amplification. Using DGGE analysis the microbial community composition, diversity, richness, carrying capacity and dynamics present in these samples of panchagavya preparation besides, individual ingredients and phylloplane samples were studied. GCMS analysis of panchagavya revealed a total of 36 compounds present in panchagavya samples. Fifteen of these compounds were reported to have biological activities such as antibacterial, antifungal, antioxidants, antitumor and nematicidal activity while the activity of the remaining 21 is yet to be known. Sequence based comparative analysis between the three stages of panchagavya at phylum level showed that Firmicutes, Actinobacteria and Proteobacteria were higher in number in 30 days old panchagavya sample. Only Bacteroidetes was observed as the highest phylum in the panchagavya sample drawn on 14h day. Bacteria belonging to unassigned genus were higher. Genera such as; Megasphaera, Lactobacillus, Bulleidia and Rumminococcus were observed in higher percentage in 30th day panchagavya sample as compared to 14th and 21st days samples. From the study it is evident that panchagavya, ingredients and phylloplane contains specific microbial community with high diversity, high carrying capacity and even distribution of species showing medium level of dynamics between the stages of panchagavya. Panchagavya sample of 21 days old appeared to have more microbial diversity than 30th day old panchagavya sample.
Subject: Biotechnology
Theme: METAGENOMIC ANALYSIS OF PANCHAGAVYA
These Type: M.Sc
Issue Date: 2015-07
Appears in Collections:Thesis

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