Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810039306
Authors: Roop Kamal
Editor: Sharma, Priti
Title: Generation and characterization of doubled haploid plants in maize
Publisher: Punjab Agricultural University, Ludhiana
Language: en
Type: Thesis
Pages: 45
Agrotags: null
Keywords: Zea mays, Haploid Induction Rate (HIR), R1-nj system, colchicine, TAIL (Tropically Adapted Inducer Lines),haploid induction system
Abstract: Doubled Haploid (DH) technology has revolutionized the production of hybrids in maize by developing homozygous inbred lines in 2-3 years which are otherwise produced in 7-8 years using conventional breeding methods. Doubled haploids are produced by using two methods viz., in vitro and in vivo methods but in vivo method is commonly used for obtaining doubled haploids in maize. In in vivo method, Tropically Adapted Inducer Lines (TAIL) hybrid was used for haploid induction. Four maize inbred lines (CM140, LM16, CM123 and I122) were used as target material for haploid induction. Crosses were made between target population to obtain F1 seeds and then crosses between F1 hybrids and inducer stock resulted in Haploid Induction Rate (HIR) that ranges from zero to 9.72 per cent. Two crosses involving CM123 as female parent did not show the expression of R1-nj system which may be due to the presence of inhibitor genes in the maternal genotypes. Haploid seeds were germinated by using two methods from which petri plate method was found to be more suitable with germination percentage range from 89.54 to 93.37 per cent. Due to more biotic stress on seedlings in Spring season, survival percentage in field was 23.37 per cent. The morphological observations of the colchicine treated plants conferred only one doubled haploid plant. Therefore, the chromosome doubling percentage worked out to be 0.42 per cent. Confirmation of doubled haploid plant was done by using cytological study and flow cytometry. So, inducer lines adapted for tropical environment should be developed to enhance the frequency of haploid induction as well as sound knowledge of genotypes regarding the presence of inhibitor genes should be explored which is of utmost importance for haploid induction.
Subject: Genetics and Plant Breeding
Issue Date: 2017
Appears in Collections:Thesis

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