Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810038703
Authors: PARVATI
Advisor: Dr. K. S. JAGADEESH
Title: EVALUATION OF ACTINOBACTERIA FOR THE BIOCONTROL OF BACTERIAL WILT OF TOMATO (Solanum lycopersicum L.) CAUSED BY Ralstonia solanacearum E. F. Smith
Publisher: University of Agricultural Sciences, Dharwad
Language: en
Type: Thesis
Agrotags: null
Keywords: EVALUATION OF ACTINOBACTERIA FOR THE BIOCONTROL OF BACTERIAL WILT OF TOMATO (Solanum lycopersicum L.) CAUSED BY Ralstonia solanacearum E. F. Smith
Abstract: As many as 35 actinobacterial isolates were screened in vitro for the inhibition of Ralstonia solanacearum causing bacterial wilt disease in tomato. Out of them, five isolates were found to be potent antagonists with the zone of inhibition (ZOI) of the pathogen varying from 19-36 mm diameter. Amongst the isolates, the isolate AUDT-47 resulted in the highest ZOI of 36 mm diameter. The actinobacteria were subjected to elucidation of their mechanisms of biocontrol. They were screened for production of antibiotics, siderophores, HCN and ISR molecules. All the five potent actinobacterial isolates such as AUDT-29, AUDT-30, AUDT-41, AUDT-47, AUDT-50 showed positive results for antibiotic production, negative results for siderophore production and were weak HCN procedures. Furthermore, they also produced ISR molecules. Increase in activity of ISR molecules viz., peroxide (PO), polyphenoloxidase (PPO), phenylalanine ammonia lyase (PALase) and total phenolic content were observed due to inoculation with these isolates. The effects of these actinobacterial isolates on growth and yield of tomato and on the incidence of bacterial wilt were studied in pot cultures. All the isolates controlled the bacterial wilt disease, with the highest per cent disease control of 80.00 per cent by AUDT- 47 isolate. Increase in the plant height and chlorophyll content were ofund to be significantly higher in the actinobacteria- treated tomato seedlings over uninoculated control. Actinobacterial inoculation increased plant biomass, lycopene content and fruit yield of tomato significantly. The highest yield was recorded by AUDT-47 isolate. The dehydrogenase activity was found to be significantly higher in the actinobacteria-treated tomato seedlings over uninoculated control. Thus, it can be concluded that the actinobacterial isolate, AUDT-47 is a superior antagonistic isolate against R. solanacearum both in vitro and in vivo.
Subject: Agricultural Microbiology
Theme: EVALUATION OF ACTINOBACTERIA FOR THE BIOCONTROL OF BACTERIAL WILT OF TOMATO (Solanum lycopersicum L.) CAUSED BY Ralstonia solanacearum E. F. Smith
These Type: M.Sc
Issue Date: 2014-12
Appears in Collections:Thesis

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