Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810036302
Authors: Parekh, V.B.
Advisor: Patel, R.M.
Title: TAGGING OF DWARF MUTANT PLANT TYPE LINKED MARKER IN THE GENOMIC BACKGROUND OF PIGEONPEA [Cajanus cajan (L.) Millsp.] THROUGH BULK SEGREGATION ANALYSIS
Publisher: DEPARTMENT OF PLANT MOLECULAR BIOLOGY AND BIOTECHNOLOGY N. M. COLLEGE OF AGRICULTURE NAVSARI AGRICULTURAL UNIVERSITY Navsari
Language: en
Type: Thesis
Pages: 95
Agrotags: null
Abstract: In the present studies, fifty crosses had been made with compact-dwarf genotype NPM2 and open-tall genotype GT-1 to obtain F1 hybrid during kharif season. The F1 hybrids seeds were sown in the field and harvested F2 seeds from the respective plants. Then, from F2 seeds, 101 plants were successfully grown for morphological and molecular analysis. The morphological characters of F2 population exhibited wide range of variability for all the characters studied than the parents. Among the morphological characters of F2 population, Correlation coefficient studied showed that the plant height had significantly negative correlation with secondary branches per plant, day to flowering and days to maturity. While, primary branches showed significantly positive correlation with secondary branches. Similarly, secondary branches observed significantly positive correlation with days to flowering and days to maturity. Days to flowering reported significantly positive correlation with days to maturity. Through bulk segregations analysis, OPA-12 and UBC-891 markers and UBC-882 and OPD-07 markers were found putatively associated for compact-dwarf specific and for open-tall specific with plant type specific character, respectively. In chi-square analysis, markers OPD07OT740 (open-tall) and UBC891CD710 (compact-dwarf) reported open-tall:compact-dwarf :: 3:1 segregation indicating the open-tall character was governed by monogenic dominant allele while, compact- dwarf character was governed by monogenic recessive allele. The chi- square analysis of F2 segregation population showed segregation pattern of the marker OPD07OT740 and UBC891CD710 was observed significantly defer from the expected 9:3:3:1 ratio, indicating the markers are linked with the plant type traits. In the linkage analysis, location of the marker UBC891CD710 observed at the distance of 10.86 ± 0.031 cM from the gene locus governing compact-dwarf plant type. Another marker OPD07OT740 was found at the distance of 66.42 ± 0.049 cM to gene locus governing open-tall plant type. Hence, UBC891CD710 reported closely linked to gene locus as compared to marker OPD07OT740. In sequencing analysis, the sequence motif of the marker UBC891CD710 reported length of read 600 bp, approximately. While marker OPD07OT740 reported the length of read 680 bp the length of read, approximately. The primer pair PP91 was amplified a compact- dwarf plant (NPM2) specific approximately 1160 bp band at 65 ºC. The SCAR marker NPM21160 was reported as dominant and also validated for compact-dwarf (NPM2) individuals.
Subject: Biotechnology and molecular Biology
Theme: Ph.D. Thesis
These Type: Ph.D
Issue Date: 2016-11
Appears in Collections:Doctoral Theses

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