Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810035625
Authors: JAYALAKSHMI K.
Advisor: Dr. S. LINGARAJU
Title: STUDIES ON LECTINS FOR SUPPRESSION OF SOIL-BORNE PATHOGENS
Publisher: University of Agricultural Science, Dharward
Language: en
Type: Thesis
Agrotags: null
Keywords: STUDIES ON LECTINS FOR SUPPRESSION OF SOIL-BORNE PATHOGENS
Abstract: Soil-borne pathogens affecting tomato (Fusarium oxysporum, Sclerotium rolfsii, Rhizoctonia solani, Ralstonia solancearum and Meloidogyne incognita) were collected from MARS, UAS, Dharwad. Expression of a fungal lectin SRL1 and a plant tuber lectin RVL1 was checked with total protein isolated from induced E. coli BL21(DE3) pLysS. E. coli induced RVL1 and SRL1 could agglutinate rabbit erythrocytes. Polyclonal antibody was raised against SRL1 by immunizing rabbits with pure antigen (SRL1) and the specificity of the antiserum (anti-SRL1) was determined by DAC-ELISA. Different concentrations of SRL1, anti-SRL1 and RVL1 were efficacious against soil-borne pathogens as demonstrated by employing different methods (poison food, spread plate, inhibition zone, spore germination inhibition). Combination of lectins with antagonists (Trichoderma spp. and Pseudomonas fluorescens strains) was found to be effective in suppressing the growth of soil-borne pathogens. But SRL1 and RVL1 suppressed some of the fungal and bacterial antagonists. Eight transgenic tomato events carrying srl1 and rvl1 genes (T3 genertaion of homozygous) showed moderately resistant reaction [SRL1-T0(21) and RVL1-T0(28)] to Fusarium, Ralstonia and also Meloidogyne and to combination of all these three pathogens. Histopathological and histochemical changes in transgenic and non transgenic tomato plants revealed reduced giant cell formation in srl1 and rvl1 transgenics. Elucidation of the mode of action of SRL1, RVL1 and anti-SRL1 on soil borne pathogens and antagonists: FITC-labelled lectins bound to hyphal wall, septum, and spores of respective fungi; localization of anti-SRL1 on sclerotia of S. rolfsii and R. solani showed a dense mass of congregated lectin sites. Nematode secretions were observed at amphidial, excretory pore and phasmidial region using CBB-R histochemical dye. Binding of lectins in nematode was found at head region, mid gut of the alimentary-tract, excretory pore which extended to the posterior region (phasmids).
Subject: Plant Pathology
Theme: STUDIES ON LECTINS FOR SUPPRESSION OF SOIL-BORNE PATHOGENS
These Type: Ph.D
Issue Date: 2014-05
Appears in Collections:Thesis

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