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Authors: Mahendra Singh
Advisor: Nanda, Trilok
Title: Molecular characterization of Indian isolates of Bovine Herpes Virus-1
Publisher: LUVAS
Language: en
Type: Thesis
Keywords: BHV-1, IBR, gB, gC, gE, PCR
Abstract: Bovine herpesvirus 1 (BHV-1) is the causative agent of a diverse clinical syndromes, including infectious bovine rhinotracheitis (IBR), infectious pustularvulvovaginitis (IPV) and infectious balanoposthitisabortion, infertility, conjunctivitis and encephalitis in bovine species (Gibbs and Rweyemamu, 1977). It is an important viral pathogen of cattle and buffalo, responsible for great economic loss to livestock sector in terms of milk production and reproduction (Gibbs and Rweyemamu, 1977). The Government of India has decided to screen breeding bulls for the BHV-1 infection before using semen for artificial insemination purposes. The seroprevalence of BHV‑1 infections has been reported repeatedly from different parts of the country (Singh et al., 2010). However, studies on PCR based screening of blood and semen of breeding bulls for BHV‑1 status are scanty. Thus the purpose of this study is to characterize the field isolate of BHV-1. The aim of the present study was to characterize the field isolates of Bovine Herpesvirus-1 (BHV-1) using gB, gC & gE gene based PCR technology as molecular diagnostic tool. Out of 187 samples, only 36 of HF cattle blood were found positive in PCR using all three sets of PCR primer. Not a single samples of buffaloe blood/semen sample was found positive for BoHV-1 using gB, gC and gE gene based PCR. On the basis of nucleotide sequence analysis, our isolates may be genotyped as BHV-1.1. Over all it can be concluded that, BHV-1 is mainly prevalent in exotic cattle in Haryana.
Subject: Animal Biotechnology
Theme: Molecular characterization of Indian isolates of Bovine Herpes Virus-1
These Type: M.V.Sc.
Issue Date: 2014
Appears in Collections:Thesis

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