Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810026461
Authors: Singh, Anjali
Advisor: Prasad, Minakshi
Title: Study of host pathogen interaction by identification of gene(s) involved in bluetongue virus infection by transcriptome analysis
Publisher: LUVAS
Language: en
Type: Thesis
Keywords: BTV, Transcriptome, Interactome, Transcription factors, Gene expression, Gene Ontology
Abstract: Bluetongue disease is an important infectious and non-contagious disease of domestic and wild ruminants. Sheep is the most susceptible host while cattle, buffalo and goats serve as reservoir. The presence or absence of clinical disease among different hosts is not clearly understood and both, the virus and host species are thought to be of importance. Therefore, in this study we investigated the host-virus interactions by infecting the peripheral blood mononuclear cells (PBMCs) isolated from sheep and goat with BTV-16 virus to quantify the global changes in the transcriptomic signature by RNA-sequencing. Comparison of infected transcriptome with control transcriptome revealed a total of 3421 differentially expressed (DE) genes in case of sheep and 4956 DE genes in case of goat. Functional analysis showed that enrichment of immune regulatory pathways under BTV infection was more in case of infected PBMCs of sheep than that of goat. The key genes involved in immune system regulation, cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, toll-like receptor signaling pathway and p53 signaling pathway were identified to be dysregulated in the infected PBMCs of both the species. On the other hand, genes involved in chemokine signaling pathway, Fc-epsilon RI signaling pathway, hemostasis and signaling in immune system were found to be enriched only in case of infected PBMCs of sheep whereas genes involved in mTOR signaling pathway, insulin signaling pathway, TGF beta signaling pathway and ATM Signaling Pathway were observed only in case of infected PBMCs of goat. Four DE genes, based on their roles in immune regulation and viral entry were validated using real time RT-PCR. The RNA seq analysis was found to coincide with the real time validation done in our experiment.
Subject: Animal Biotechnology
Theme: Study of host pathogen interaction by identification of gene(s) involved in bluetongue virus infection by transcriptome analysis
These Type: M.V.Sc.
Issue Date: 2014
Appears in Collections:Thesis

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