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|Title:||In vitro establishment and morphogenetic study in litchi (Litchi chinensis Sonn.) cv. Purbi|
|Publisher:||Department of Horticulture (Fruit & Fruit Technology), BAU, Sabour|
|Keywords:||Micropropagation, shoot proliferation, callus induction|
|Abstract:||Conventionally litchi is propagated by vegetative means mainly by air layering or marcottage, grafting and budding. Although various means to increase the efficiency of these methods have been tried such as by use of younger branches, small earth balls & 1,4-indole-3-butyric acid (IBA), the process is sill slow and inefficient. Hence for large scale production of elite litchi clones, micropropagation can be used as a potential alternative to conventional means of reproduction. It holds a great potential as an efficient propagation technique to meet the increasing demand of quality planting material free from diseases and pest and can play a significant role in crop improvement. Keeping in view these factors this investigation was carried out. One of the most commonly encountered problems in in vitro culture establishment is the contamination. The efficiency of sterilizing agents was evaluated in terms of maximum aseptic live explants. When no sterilants was used all the explants were contaminated. The contamination significantly decreased with the increase in concentration of sterilants and their time of exposure. The presence of phenolic compounds causing death of explants has been one of the major bottlenecks of litchi micropropagation. In our experiment, all the antioxidants treatments significantly reduced phenolic exudation. However, treatment with combination of ascorbic acid and citric acid (150 mg/l each) to the explants and ascorbic acid (300 mg/l) to the media recorded almost no browning of either of explants. Addition of cytokinins alone or in combination with auxin was found essential during initiation and establishment of cultures. In nodal segment maximum per cent shoot proliferation and shoot with basal callus (36.4±0.48) in MS and (37.6±0.51) in WPM media was observed with the treatment of 2.5 mg/l BAP alone in media, whereas treatment combination of 1.0 mg/l IAA & 5.0 mg/l BAP resulted in maximum number of shoots (3.0±0.26) in MS and (3.2±0.29) in WPM media. Although for leaf explants, maximum per cent callus induction (54.1±0.20) in MS and (49.8±0.19) in WPM media was recorded, when 2.5 mg/l 2,4-D was used. However, highest per cent callus forming shoots (49.9±1.16) in MS and (47.2± 1.07) in WPM media resulted with 2.5 mg/l IAA and 2.5 mg/l BAP in combination. Treatment with only 2.5 mg/l BAP alone recorded maximum number of shoots (4.0±0.11) in MS and (3.8±0.11) in WPM media through leaf callus. It was also found that WPM proved to be better media for shoot proliferation in nodal segment whereas MS media for callus induction. Litchi is a prominent crop of Bihar and therefore further research needs to be conducted to study all the factors involved in shoot regeneration, multiplication and acclimatization to bring better results.|
|Appears in Collections:||Theses|
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