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Authors: Kanika Rani
Advisor: Jain, R.K.
Title: Phenotyping and molecular marker analysis of selected F3 segregating lines from aerobic x low land indica rice (Oryza sativa L.) crosses
Publisher: CCSHAU
Language: en
Type: Thesis
Agrotags: planting, rice, Vegetative propagation, Grain, Yields, Irrigation, Developmental stages, Alleles, Genotypes, Land resources
Abstract: Experiments were conducted to evaluate F3 segregating populations derived from the five crosses (PAU201 x MAS25, MASARB25 x PAU201, PAU201 x MAS26, MASARB25 x HKR47 and MAS25 x HKR47) for various physio-morphological and/or root traits and microsatellite markers linked to the traits promoting aerobic adaptation. MASARB25, MAS25 and MAS26 are aerobic while PAU201 and HKR47 are low-land indica rice varieties. In all the five populations, wide variation was observed for plant height, panicle length, number of panicles per plant, number of effective tillers per plant, root length, root thickness, fresh and dry root weight, 1000 grain weight, grain length-breadth ratio and grain yield per plant. In these populations, significant positive correlation was observed between yield per plant with plant height, effective no. of tillers per plant, length-breadth ratio, 1000 grain weight, root biomass and/or root length. The NTSYS-pc UPGMA tree cluster analysis and 2-D PCA scaling of selected F3 plants derived from the five crosses clearly showed large variation among two parental genotypes and F3 plants were invariably interspersed between them. A number of promising F3 plants have been selected, which had higher grain yield, root length and biomass greater than MAS25, MAS26 and MASARB25 for further progeny analysis. Most of these selected plants had the desired allele for the markers reported earlier to be linked with the aerobic adaptation traits (RM234 and RM547 for root length on chromosome 7 and 8 respectively; RM525 for root thickness and root biomass on chromosome 2).
Subject: Molecular Biology & Biotechnology
These Type: Ph.D
Issue Date: 2015
Appears in Collections:Theses

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