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Title: Refinement of technology for micropropagation of carnation (Dianthus caryophyllus L.) cv. ‘Master’
Publisher: UHF,NAUNI
Language: en
Type: Thesis
Agrotags: null
Keywords: carnation (Dianthus caryophyllus L.) cv. ‘Master,micropropagation
Abstract: ABSTRACT The present investigation aims at “Refinement of technology for micropropagation of carnation (Dianthus caryophyllus L.) cv. ‘Master’”. Surface sterilized shoot buds cultured on control (MS + 3% sucrose + 0.8% agar-agar) supplemented with 2.0 mg/l BA which showed 77.77% establishment. Best medium for multiplication was MS medium supplemented with 2.0 mg/l BA producing 4.50 number of microshoots of shoot length of 2.44 cm, but as it resulted in hyperhydricity of shoots so medium supplemented with kinetin (2.0 mg/l) was used which produced 3.67 number of microshoots and 4.55 cm shoot length. Low cost medium containing 30 g/l table sugar, 50 g/l starch and aquaguard water produced 3.52 shoots with 3.01 cm shoot length. It was also observed that subculturing passage affected the average number of shoots per explant and also the shoot length which was highest in case of fifth subculturing resulting in 5.58 and 5.27 number of shoots per explant on control and low cost medium and shoot length of 4.66 cm and 3.53 cm , respectively. 62.49% rooting and 4.50 average roots per shoot with 1.61cm was found on 1/2 MS + 0.04% activated charcoal and 48.58% rooting with 3.50 average roots per shoot and 1.75 cm was observed on 1/2 MS + 50g/l starch + 0.04% activated charcoal + 15 g/l table sugar. The study has resulted in the reduction cost of the medium by substituting agar-agar and sucrose with starch and table sugar.
Subject: Biotechnology
Theme: carnation (Dianthus caryophyllus L.) cv. ‘Master,micropropagation
These Type: M.Sc
Issue Date: 2015
Appears in Collections:Theses

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