Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810010440
Authors: Saurabh Singh Singhal
Advisor: Prof. (Mrs.) Nalini Kataria
Title: CORRELATION OF ERYTHROCYTE ANTIOXIDANTS AND IRON INDICES IN INDIGENOUS PIG FROM ARID TRACTS DURING EXTREME AMBIENCES
Publisher: Rajasthan University of Veterinary and Animal Sciences Bikaner-334001
Language: en
Type: Thesis
Agrotags: null
Abstract: An endeavour was launched to correlate the erythrocyte antioxidants and iron indices in indigenous pig from arid tracts during moderate, hot and cold ambiences. Blood samples were collected to harvest plasma and erythrocytes from apparently healthy male indigenous pigs during slaughtering from private slaughter houses in and around Bikaner district during moderate, extreme hot and extreme cold ambiences. The male pigs were grouped as 4-6 months; 6-8 months; 8-10 months and 10-12 months of age groups in each ambience. To accomplish the objectives, non-enzymatic and enzymatic antioxidants in the erythrocytes; plasma iron indices and total plasma proteins were determined. Non-enzymatic antioxidants incorporated vitamin A, vitamin C, vitamin E and glutathione and enzymatic antioxidants incorporated superoxide dismutase, glutathione reductase, catalase and peroxidase. Indices of iron in plasma included total iron, total iron binding capacity and transferring saturation. The moderate mean overall values of erythrocytic indices i.e. vitamin A (µmol gHb-1),vitamin C (µmol gHb-1),vitamin E(µmol gHb-1), glutathione (µmol gHb-1), superoxide dismutase (kU gHb-1), glutathione reductase (kU gHb-1), catalase (kU gHb-1) and peroxidase (mU gHb-1),and plasma indices i.e. total iron (µmol L-1), total iron binding capacity(µmol L-1), transferrin saturation (%) and total proteins (g L-1) were 0.52±0.10; 2.74±0.01; 0.56±0.04; 2.39±0.008; 3.63±0.12; 1.72±0.09; 2.74±0.03; 7.57±0.08; 31.01±0.33; 65.816±0.14; 47.04±0.71 and 74.00±0.30, respectively. The mean values of erythrocytic non-enzymatic antioxidants; plasma iron indices and plasma total proteins were found significantly (p≤0.05) lower during hot and cold ambiences as compared to respective moderate overall mean value. It was found that decline in the values during hot ambience was greater than that of cold ambience. The mean values of erythrocytic enzymatic antioxidants were found significantly (p≤0.05) higher during hot and cold ambiences as compared to respective moderate overall mean value. It was found that increase in the values during hot ambience was greater than that of cold ambience for each enzymatic indicator of antioxidant status. Age effect showed a significant (p≤0.05) increase in the mean values in each ambience being highest in the pigs of 10-12 months of age and lowest in pigs of 4-6 months of age for all the indicators studied in the present endeavour. Effect of extreme hot and cold ambiences were evident on each group. Outcome of the present endeavour signified the correlation among erythrocyte antioxidants, plasma iron indices and total proteins during extreme hot and cold ambiences in indigenous pig. The correlations among non-enzymatic antioxidants, plasma iron indices and plasma proteins; and among enzymatic antioxidants were highly significant (p≤0.01) and positive. The correlations between enzymatic indicators of antioxidant status and other variables were highly significant (p≤0.01) and negative. It can be concluded that extreme hot and cold ambiences affected the erythrocytic antioxidant status along with iron indices and total proteins in indigenous pigs of all age groups from arid tracts. Magnitude of impact was greater during hot ambience than cold ambience. Indigenous pigs of 4-6 months of age were affected with greater impact than other age groups. This advocated the fact that pigs of all age groups were influenced owing to dire variations in ambient temperatures during hot and cold ambiences. Maximum per cent variations were observed in the mean values of superoxide dismutase during hot ambience and glutathione reductase mean values during extreme cold ambience. Comparatively higher values of superoxide dismutase and glutathione reductase are known to be the most important components of strategies of physiology of animals to combat the effects of extreme ambiences. Manner of changes of data implied the line of approach in physiological tactics by pigs during the periods when possible development of oxidative stress was there. The data collected can be used in veterinary clinical physiology for future research in the dome of antioxidant status and in arranging regulatory approaches during harsh ambiences. The observations obtained ascertained that significant correlations existed among erythrocytic antioxidants, iron indices and protein levels pointing out towards the presence of oxidative stress and they may append towards the use of appropriate contrivances to identify the injurious influences of changes in ambient temperatures on the indigenous pigs. The upshots of endeavour will immensely contribute in monitoring the health status of indigenous pigs to improve indirectly the economical status of marginal pig raisers and in research carried out to provide scientific execution of management practices to enhance the physical conditions of indigenous pigs from arid tracts.
Subject: Veterinary Physiology
Theme: CORRELATION OF ERYTHROCYTE ANTIOXIDANTS AND IRON INDICES IN INDIGENOUS PIG FROM ARID TRACTS DURING EXTREME AMBIENCES
These Type: M.V.Sc.
Issue Date: 2016
Appears in Collections:Thesis

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