Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810010298
Authors: Shukla, Vandana
Advisor: Kotasthane, A.S.
Lakpale, N.
(Smt) Ganguli, Jayalaxmi
Saxena, R.R.
Title: “CHARACTERIZATION OF FLUORESCENT PSEUDOMONAS COLLECTED FROM DIFFERENT GEOGRAPHICAL LOCATIONS OF CHHATTISGARH FOR ITS ANTAGONISTIC AND PLANT GROWTH PROMOTION ACTIVITY”
Publisher: Indira Gandhi Krishi Vishwavidyalya, Raipur
Language: en
Type: Thesis
Pages: 214 p.
Agrotags: null
Keywords: CHARACTERIZATION, FLUORESCENT PSEUDOMONAS, GEOGRAPHICAL LOCATIONS, CHHATTISGARH, ANTAGONISTIC, PLANT GROWTH PROMOTION ACTIVITY
Abstract: The present investigation was carried out in Molecular Plant Pathology Laboratory at the Department of Plant Molecular Biology and Biotechnology, College of Agriculture, IGKV, Raipur to isolate and characterise fluorescent Pseudomonas. The plant rhizosphere is an important soil ecological environment for plant microbe interactions. It involves colonization by a variety of micro-organisms in and around the roots which may result in symbiotic, associative, neutralistic or parasitic relations within the plant, depending on the type of microorganism, soil nutrient status, plant defense system and soil environment. A substantial number of bacterial species, mostly those associated with rhizosphere, may exert a beneficial effect on plant growth and development. Plant growth promoting rhizobacteria (PGPR) can stimulate growth by one or more different mechanisms. The direct mechanisms include production of growth hormones like IAA, phosphate-solubilization and uptake of iron, whereas indirect mechanisms include check on phytopathogens by the release of HCN, antibiotics and siderophores. Among PGPR, fluorescent pseudomonads (FLPs) possess several properties best suited for survival and colonization in the rhizosphere environment. Fluorescent pseudomonads are ubiquitous bacteria that are common inhabitants of the rhizosphere and are the most studied group within the genus Pseudomonas. Bacterial isolates from the rhizospheric region were assessed as potential biocontrol and PGPR agent in this study. In present study the isolates were collected from different geographical locations of Chhattisgarh and characterized based on biochemical tests. Twenty candidate isolates selected for pot and field experiments screened for phosphate solubilisation, siderophore production, ACC deaminase enzyme production and other plant growth promoting potentials. All the isolates were characterized at molecular level based on 16S rRNA gene and other gene specific primers. In present study all isolates show positive response for oxidase test, catalase test, citrate utilization and phosphate solubilisation and showed variability for other traits. In all, 20 bacterial isolates were found best with antifungal activity against Sclerotium rolfsii. Of 20 isolates, 4 were selected according to their high efficiency in in vitro antagonism. Plant growth promoting response of different crops was observed following seed bacterization with fluorescent Pseudomonas (expressing different levels siderophore) isolates. We were able to identify potential candidate isolates for their ability to produce siderophore, phosphate solubilization, IAA producing and plant growth promoting ability. Isolates P66, P-10, P-12, P-22 and P-30 were identified as potential antagonists in-vitro against S. rolfsii and potential PGPR agent. Molecular characterization of 20 isolates of fluorescent Pseudomonas resolved homogeneity with ITS primer and a high level of polymorphism was seen with ERIC, REP and BOX primers.
Subject: Plant Pathology
Theme: Agriculture, Plant Pathology
These Type: Ph.D
Issue Date: 216
Appears in Collections:Theses

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