Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810009925
Authors: MISHRA, CHANDRAPRAKASH SIYARAM
Advisor: Ghosh, R. C.
Title: Studies on Toxicopathology of Acephate In Wistar Rats and its Amelioration with Cow and Goat Urine distillates
Publisher: Chhattisgarh Kamdhenu Vishwavidyalaya, Durg
Language: en_US
Type: Thesis
Agrotags: null
Abstract: The present investigation was undertaken to study the toxicopathology of Acephate in Wistar Rats and its amelioration with Cow and Goat Urine distillates. The approximate lethal dose of Acephate was determined as 1350 mg/kg body weight. The present study of acute toxicity clinically revealed scanty loose dropping, profuse salivation, bloody tears, depression, prostration, hyperaesthesia, respiratory distress, exophthalmos, motor incoordination, soiled perineum, loss of all reflexes and death. At necropsy, there was congestion in most of the visceral organs. Histopathologically, Acephate intoxicated rats revealed haemorrhages, degenerative and necrotic changes in liver, kidney, heart and lungs. There was thickening of the interalveolar septa in lungs, severe depletion of lymphocytes in the Malpighian corpuscles of the spleen and moderate degree of degenerative and necrotic changes in brain. In the 28 days subacute study, total number of one hundred and ninety two rats were divided at random into twelve equal groups of 16 each (8 males + 8 females). All the rats of groups IV, V and VI were administered Acephate orally @ 60, 90 and 135 mg/kg body weight respectively for 28 days and all the rats of groups VII, VIII and IX were administered Acephate orally @ 60, 90 and 135 mg/kg body weight along with cow urine distillate @ 1 ml/100 g body weight for 28 days respectively. All the rats of groups X, XI and XII were administered Acephate orally @ 60, 90 and 135 mg/kg body weight along with goat urine distillate @ 1 ml/100 g body weight for 28 days respectively. Rats of group I served as normal control, rats of group II as a cow urine distillate control and rats of group III as a goat urine distillate control. In subacute toxicity, mean body weight was significantly (P<0.01) reduced in dose dependant manner in the intoxicated rats of group IV, V and VI, while administration of cow and goat urine distillate resulted in increase in body weight. Acephate caused significant (P<0.01) increase in the OSI of liver and kidneys of rats of groups V and VI and significant (P<0.01) decrease in the OSI of spleen and testes of groups V and VI . Administration of cow and goat urine distillate significantly reversed the OSI index in rats. In Acephate intoxication, haematological examination revealed that the TEC, TLC, Hb, and PCV were significantly (P<0.01) decreased and MCV and neutrophil % significantly increased in rats of group VI. Amelioration with cow and goat urine distillate resulted in improvement in blood parameters. Biochemically the values of AST, ALT, ALP, BUN, creatinine, uric acid and glucose were significantly (P<0.01) increased while values of mean total protein, albumin, cholesterol, triglycerides, acetylcholinesterse, calcium and phosphorous significantly (P<0.01) decreased with the increase in dose of Acephate. The values of AST, ALP, BUN, uric acid and glucose were significantly (P<0.01) decreased in cow and goat urine distillate treated rats of groups IX and XII. Administration of cow and goat urine distillate significantly (P<0.01) reversed the cholinesterase inhibition in rats and resulted in significant (P<0.01) recovery. . Evaluation of CMI response against challenge dose of DNFB was significantly (P < 0.01) decreased in groups IV, V and VI. Whereas cow and goat urine distillate treated rats of groups VIII, IX and XII showed significant (P<0.01) amelioration in CMI response. Evaluation of male reproductive indices also showed significant (P ≤ 0.01) decrease in epididymal sperm concentration, sperm motility, live spermatozoa % along with an increase in dead spermatozoa % and spermatozoa abnormalities in rats of group treated with Acephate whereas cow and goat urine distillate treated rats of groups VIII, IX, XI and XII showed significant (P ≤ 0.01) amelioration in all reproductive parameters. Gross lesions revealed congestion, haemorrhages and enlarged liver , pale and enlarged kidneys, congestion, haemorrhages in lungs, slight atrophy of spleen and testis and mild congestion in brain. Histopathological lesions varied in a dose dependent manner. Mild, moderate and severe degenerative and necrotic changes and congestion/haemorrhage were observed in liver, kidney, heart, lungs,brain and testis and of rats of groups IV, V and VI respectively indicating Acephate induced hepatotoxicity, nephrotoxicity, immunosuppression, neurotoxicity and also male reproductive toxicity. Mild to severe lymphocytolysis were observed in Malpighian corpuscles of spleen in rats of groups IV, V and VI indicating immunotoxicity of Acephate. Thus both cow and goat urine distillate showed amelioration of Acephate toxicity in wistar rats but the amelioration was comparatively better in rats of cow urine distillate group than the rats of goat urine distillate group.
Subject: Veterinary Pathology
Theme: Studies on Toxicopathology of Acephate In Wistar Rats and its Amelioration with Cow and Goat Urine distillates
These Type: Ph.D
Issue Date: 2014
Appears in Collections:Theses

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