Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810004409
Authors: BHARAT, Mr. MORALE SIDHESHWAR
Advisor: Mate, Dr.S.N
Manjare, Dr.M.R
Selar, Dr.V.R
Kale, Dr.A.A
Title: MORPHOLOGICAL, CHEMICAL AND BIOCHEMICAL CHARACTERIZATION OF PEARLMILLET [Pennisetum glaucum (L.) R.Br.] HYBRIDS AND THEIR PARENTAL LINES
Publisher: MAHATMA PHULE KRISHI VIDYAPEETH RAHURI-413 722, DIST. AHMEDNAGAR MAHARASHTRA STATE (INDIA)
Language: en
Type: Thesis
Agrotags: null
Abstract: The present investigation entitled, "Morphological, chemical and biochemical characterization of pearlmillet hybrids and their parental lines" was undertaken during 2006-07 at Post Graduate Institute Farm, Department of Botany and Seed Technology Research Unit, Mahatma Phule Krishi Vidyapeeth, Rahuri (Maharashtra). The experimental material consisted of six hybrids and their parental lines. The observations were recorded on seed, seedling and plant morphological characters, besides chemical and electrophoretic tests. The morphological characteristics exhibited by different genotypes studied indicated that although some of the genotypes have common morphological features in respect of one or few characters, they can be differentiated from each other on the basis of other characters. Discrimination among genotypes was possible on the basis of seed characteristics exhibited by them. Mainly three seed characters were studied viz., seed size, seed colour and seed shape. The genotypes RHRB-13A and RHRB- 14B exhibit small seed size and all other varieties of bold and medium seed size. Further, differentiation was on the basis of seed shape and seed colour. Three different seed shapes were observed i.e. obovate, globular and hexagonal and four seed colours were observed i.e. grey, deep grey, yellow brown and grey brown. Most of genotypes were obovate in shape and deep grey in colour. The hybrids and their pa rental lines showed variations for all the 26 plant morphological characters studied. The characterization of hybrids and their parental lines can be done on the basis of morphological characterization supplemented with the differences recorded in genotypes by using chemical and biochemical tests. In pearlmillet mainly peroxidase enzyme test, phenol test and GA3 test were carried out. On the basis of degree of darkening of colour on the seeds, genotypes were grouped into different categories and in GA3 test on the basis of higher growth over water treated control genotypes were grouped as high responsive and low responsive. The electrophoresis was carried out into 2 groups, each group consisting of nine genotypes. The number of bands ranged between 5 to 10 with total thirty number of bands in group first while bands ranged between 6 and 9 with total twenty seven number of bands in group second. Each cultivar can be differentiated from each other with respect to presence or absence of a particular band. From the present study, it can be concluded that some of the morphological characters coupled with chemical tests could be exploited for distinguishing varieties in different groups. However, electrophoresis gives more precise results for differentiation of pearlmillet genotypes, being stable and not influenced by the environments.
Subject: Seed Science and Technology
Theme: SEED SCIENCE AND TECHNOLOGY
These Type: M.Sc
Issue Date: 200-05-28
Appears in Collections:Thesis

Files in This Item:
File Description SizeFormat 
19_C.pdf8.25 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.