Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810002632
Authors: MADHUKAR, MAHALE SHRIKANT
Advisor: Karale, Dr.A.R
Patil, Dr.S.C
Kamble, Dr.A.B
Kaledhonkar, Prof.D.P
Title: STUDIES ON IN VITRO PROPAGATION OF TAMARIND (Tamarindus indica L.)
Publisher: MAHATMA PHULE KRISHIVIDYAPEETH, RAHURI-413 722, DIST. AHMEDNAGAR MAHARASHTRA STATE (INDIA)
Language: en
Type: Thesis
Agrotags: null
Abstract: The present study entitled, "Studies on in vitro propagation of tamarind {Tamarindus indiaq.L.)" was conducted during 2002-2003 with a view to study the influence of month (season) of explant collection on bud break, to standardize the nutrient medium for in vitro shoot regeneration and multiplication and to study the in vitro performance of certain elite types of tamarind viz., Sweet pulped type (Thailand), Sour pulped type and Red pulped types. Axillary buds from mature trees of three genotypes were used as an explant. Murashige and Skoog (MS) medium with various combination of 6-benzylamino purine (BAP) with 1-napthalene acetic acid (NAA) and Gibberellic acid (GA3) was tried for each type of genotype. The results -were analysed by adopting completely randomized block design. From the present study, it can be seen that treatment MS + BAP 2.0 mg l-1 + GA3 0.5 mg l-1 + NAA 0.1 mg l-1 gave optimum and consistent results with respect to per cent bud break and number of days required for bud break in in case of all three genotypes. But intervarietal differences in response to growth regulators were observed. However, sprouted shoots showed abscission of leaflets and death of the culture. Also brown callusing was observed at the base of the explant in all three genotypes. Plant growth regulator combinations tried for shoot regeneration and multiplication gave unsatisfactory results. Only brown callusing of explant was observed instead of regeneration and multiplication. In case of genotypes Sweet pulped type (Thailand) and Red pulped type bud break was recorded during February, March, April, May and June while in case of sour pulped type bud break was recorded during April, May and June. As nodal segment and shoot tip explants failed to regenerate and multiply, further investigation to study rhizogenesis could not be carried out.
Subject: Horticulture
Theme: Horticulture
These Type: M.Sc
Issue Date: 2003-10-21
Appears in Collections:Thesis

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