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|Authors:||Patil, Vdayshinh Shamrao|
Kaulgud, Prof S.N
|Title:||CHARACTERIZATION OF POMEGRANATE (Punica granatum L.) CULTIVARS BY MORPHOLOGICAL AND ELECTROPHORETIC STUDIES|
|Publisher:||MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722|
|Abstract:||The present investigation entitled "Characterization of pomegranate cultivars by morphological and electrophoretic studies" was carried out with main objectives of to characterize pomegranate cultivars based on their morphological features and banding pattern of soluble proteins through SDS-PAGE. The work was carried out at Instructional-cum-Research Farm and Tissue Culture Laboratory of Centre For Advanced Studies in Horticulture (Fruits), Department of Horticulture, M.P.K.V., Rahuri during the year 1998-99. The deciduous nature of foliage, erect growing habit, deep green colour of the new flush, pale red colour of ovary and characteristic red colour of fruits were the diagnostic characters to isolate the cultivar Gul-e-shah. Spinous nature was an important character to differentiate the various cultivars. Cultivars Ganesh and Jodhpur Red were profusely spiny than rest of the cultivars. Dholka could be identified from the other cultivars on the basis of orange red colour of the corolla. Jodhpur Red can be differentiated from the other cultivars by the presence of flat round shaped fruits. The presence of distinct claw in the corolla isolates cvs. Ganesh and P-13 from other cultivars. Straw yellow colour of style and ovary was the unique characteristic of cv. Ganesh. Newly developed cultivar Mridula can be characterized by the colour of petiole, the characteristic capsicum red colours of inner surface and cardinal red colour of outer surface of calyx, blood red colour of corolla, orange red colour of style and ovary and dark red coloured fruits. Suckering habit, internodal length, arrangement of leaf, adoxidal and abaxial leaf colour, leaf shape, number of stamens, number of sepals and petals showed only slight variation between the cultivars and hence these characters were not useful to characterize the cultivars. The bark and rind of pomegranate contain poor quantity of proteins. Qualitative and quantitative variations were observed in the banding patterns of soluble seed proteins of the cultivars under study during electrophoresis. The presence or absence of some unique bands distinguish cultivars from each other viz., cv. P-13 by the presence of band Nos. 23 and 27, cv. Gul-e-Shah by band No. 37, cv. Mridula by 5 and cv. Co.White by the presence of band Nos. 14 and 18. Contrary to this cvs. Muscat and P-13 by the absence of band Nos. 22 and 26 while cvs. Gul-e-Shah and Co.White by the absence of band No. 32. The maximum similarity index value was observed among Dholka vs. Mridula showing close olutinonary relationship whereas minimum similarity index value was between cvs. Co.White vs. Dholka. As the protein banding pattern was not so discriminative enough to distinguish all the cultivars studied, there is further need of going for isoenzyme electrophoresis.|
|Appears in Collections:||Thesis|
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