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|Authors:||Fattepurkar, Sameer Chandrakant|
Kale, Dr. S.V.
|Title:||NUTRITIONAL EVALUATION OF THE GRAINS AND LEAF NITRATE REDUCTASE ACTIVITY IN PIGEONPEA GENOTYPES|
|Publisher:||MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI - 413 722, DIST. AHMEDNAGAR MAHARASHTRA, INDIA|
|Agrotags:||sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas|
|Abstract:||The present investigation aims at evaluating theproximate composition of grains of seventeen pigeonpea (Cajanus cajan L.) genotypes. In addition, the nitrate reductase (NR) activity from the trifohate leaves of pigeonpea plants was standardized by an in vivo method. The nitrate reductase activity in the leaves ofseventeen pigeonpea genotypes was also examined and correlatedwith gram protein contents. Further, it was desired to know thedistribution of NR activity in different plant parts such as leaves, stems, petioles, roots and root nodules in 45-day-old pigeonpea\"\ Abstract contd S.C. Fattepurkarplants. The pigeonpea plants were grown in soil-filled pots undernatural daylight conditions during the months from June to August 2001.Grains of seventeen pigeonpea genotypes were analysedfor biochemical parameters viz., moisture, ash, crude protein, crudefat, crude fibre, total arbohydrates and limiting amino acids such asmethionine and tryptophan. The grains of all the cultivars differedsignificantly in their contents of moisture (7.82 to 12.38 %), ash (2.89to 5.96 %), crude protein (16.93 to 26.92 %), crude fat (1.67 to 2.91 %),crude fibre (5.84 to 11.23 %), carbohydrates (51.84 to 60.79), methionine (0.80 to 1.16 g 16 g-1 N) and tryptophan (0.68 to 1.09 g 16g-1 N).The infiltration media (5.0 ml) used for the determinationof in vivo NR activity for plus KNO3 assays in pigeonpea trifoliateleaves were standardized in respect of nitrate (100 mM), n-propanol,(1 %, v/v) and Triton-X-100 (0.05 %, v/v). The diurnal variability wasobserved in NR activity in the trifoliate leaves of pigeonpea. Theaverage NR activity was low at 8.00 a.m. in the morningl^ncreasedrapidly as the day advanced, until it reached a maximum level at 2.00p.m. The NR activity, however, declined during the afternoon ours,reaching to a minimal level at 6.00 p.m. The enzymatic potentials ofXVIAbstract contd S.C. FattepurkarNR from different leaves of 21-day-old plants indicated that thehighest NR activity was observed in the cultivar ICPL-87, while thelowest was observed in ICPL-87119. The cotyledonary leaves had thehighest NRA in all the cultivars investigated. The significant positivecorrelation between NR activity in different leaves and proteincontent in the grains of the pigeonpea genotypes indicated that thegenotypes with higher protein content and NR activity such asICPL-87 and PT-11-39 can be selected for further varietalimprovement programmes. The highest NR activity was largelyconfined to petioles in 45-day-old pigeonpea plants indicating thatpetioles are the major site of nitrate reduction and ssimilation inpigeonpea, which was followed by leaves and stems. The roots androot nodules, however, had much lower level of NR.|
|Appears in Collections:||Thesis|
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