Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810001024
Authors: VASAVE, GULABSING KIRTA
Advisor: Munjal, Dr.S.V
Kute, Prof.L.S
Mane, Prof.L.L
Title: NUTRITIONAL EVALUATION OF GRAINS AND LEAF NITRATE REDUCTASE POTENTIAL OF SOME PIGEONPEA GENOTYPES
Publisher: MAHATMA PHULE KRISHI VIDYAPEETH, RAHURI -413 722, DIST.AHMEDNAGAR, MAHARASHTRA, INDIA
Language: en
Type: Thesis
Agrotags: sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas
Abstract: The present investigation involves the nutritional evaluation of grains of different maturity group pigeonpea [Cajanus cajan (L.) Millsp.] genotypes. In addition, nitrate reductase (NR) assay from the trifoliate leaves of pigeonpea plants was standardized by an in vivomethod. The nitrate reductase enzymatic potential in the leaves of fourteenpigeonpea genotypes was also examined and correlated with grain proteincontents. Further, it was desired to know the distribution of NR activity indifferent plant parts such as leaves, stems, petioles, roots and root nodulesin 45-day-old pigeonpea plants. The pigeonpea plants were grown in soilfilled earthen pots under natural daylight conditions during the monthsfrom August to October, 2002.XVU1Abstract contd... G.K. VasaveThe grains of fourteen pigeonpea genotypes were analysed forvarious biochemical constituents,such as ash, crude protein, crude fat,crude fibre, total carbohydrates and limiting amino acids such asmethionine and tryptophan. The grains of all the genotypes differed significantly in their contents of moisture (7.21 to 10.17%), ash (3.19 to5.67%), crude protein (17.59 to 26.91 %), crude fat (1.86 to 2.83%), crude fibre (4.17 to 6.66%), total carbohydrates (51.11 to 61.02%), methionine(0.80 to 1.22 g 16 g"1 N) and tryptophan (0.60 to 1.09 g 16 g"1 N).The infiltration media (5.0 ml) used for the determination ofin vivo NR activity for + KNO3 assays in pigeonpea trifoliate leaves werestandardized in respect of nitrate (100 mM), n-propanol, (1%, v/v) andTriton-X-100 (0.05%,v/v). The diurnal variability in NR activity of trifoliate leaves was also bserved. The average NR activity was low at 6.00 a.m. in the morning which increased rapidly as the day advanced,until it reached a maximum level at 2.00 p.m. The NR activity, however,declined during the afternoon hours, reaching to a minimal level at 6.00p.m. The enzymatic potential of NR from different leaves of 21-day-oldplants indicated that the highest NR activity was observed in the genotype, ICPL-87, while the lowest in PT-1037. The cotyledonary leaves had thehighest NR activity in all the genotypes investigated. The significantpositive correlation between NR activity indifferent leaves and proteincontent in the grains of the pigeonpea genotypes indicated that theXIXAbstract contd... G.K. Vasavegenotypes with higher protein content and NR activity such as ICPL-87,MDRL-14 and MDRL-6 can be selected for further varietal improvementprogrammes. The highest NR activity was largely confined to petioles in 45-day-old pigeonpea plants indicating petioles as the major site of nitratereduction and assimilation, which was followed by leaves and stems. Theroots and root nodules, however, had much lower NR level.
Subject: Agricultural Biochemistry
Theme: Agricultural Biochemistry
These Type: M.Sc
Issue Date: 2003-10-17
Appears in Collections:Thesis

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