Please use this identifier to cite or link to this item: http://krishikosh.egranth.ac.in/handle/1/5810001017
Authors: WAMAN, SANJAY LAZMAN
Advisor: Munjal, Dr. S.V
Desai, Dr.B.B
Deore, Prof. B.P
Dhage, Dr. A.R
Title: ASSESSMENT OF NITRATE REDUCTASE ACTIVITY AND CARBON MONOXIDE SENSITIVITY OF CYTOCHROME c OXIDASE IN THE LEAFLETS OF SOME CHICKPEA CULTIVARS
Publisher: MAHATMA FIHIU1LI EMUM fEWAFIITH 1AHU1MS fit CMAMAlASHTlRAIi
Language: en
Type: Thesis
Agrotags: sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas
Abstract: Chickpea seedlings were grown in soil-filled pots under green house conditions of the Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri , during the months of November, 1993 to March, 1994. The experiments were carriedout for the determination of leaflet nitrate reductase activity by an in vivo method and to monitor the varietal differences ifany in the activity of leaflet NR. Carbon monoxide sensitivityof cytochrome c oxidase with one min sparging of CO through theleaf segments/at defined C0:02 ratio^ The effect of 2,4-dinitrophenol (2,4~DNP) on CO-sensitivity in the leaflets ofsome chickpea cultivars was also monitored.xiv The in vivo NR assays for chickpea leaflets were optimized in terms of substrate and n-propanol concentrationsto be added in the infiltration media. The ptimumconcentration of KN03 to be added into the infiltrationmedia, required for maximum in vivo NR activity, was found tobe 100 mM in chickpea leaflets. It was further noticed that 4 per cent (v/v) n-propanol was optimum in the infiltration mediaused for the determination of in vivo NR activity. Diurnal fluctuations were observed in chickpea leaflet NR. The average NR activity which was low at 8.00 hr the morning increased rapidly as the day advanced until it reached a maximum level at 14.00 hr. The NR activity, however, declined during theafternoon hours and reached a minimum level in the evening at17.00 hr. Eight cultivars of chickpea were examined forcarbon monoxide sensitivity of cytochrome c oxidase by spargingCO for one min through weighed leaflet materials. All the cultivars were found to be completely insensitive to CO by thistechnique. Further a C0:02 ratio of 40 was required to inhibit cytochrome c oxidase in chickpea leaflets. Pretreatment ofleaves of six chickpea cultivars with 2 mM concentration of 2,4-DNP resulted in the ready response of leaf materials at a C0;02 ratio of 10 and even at 5.
Subject: Agricultural Biochemistry
Theme: Agricultural Biochemistry
These Type: M.Sc
Issue Date: 1994-10-18
Appears in Collections:Thesis

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