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|Authors:||Chable, Abhijeet Ramaling|
|Title:||STUDIES ON CARBON MONOXIDE SENSITIVITY OF CYTOCHROME OXIDASE AND GIBBERELLIN SENSITIVITY OF SOME CYTOPLASMIC MALE STERILE (CMS) LINES Of SORGHUM|
|Publisher:||MAHATMA PHULE KRISHI VIDYAPEETH RAHURI, 413 722 DIST.- AHMEDNAGAR. Maharashtra State (India)|
|Agrotags:||sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas|
|Abstract:||Thirteen sorghum lines were examined for carbon monoxide sensitivity of cytochrome c oxidase by an in vivo aerobic assay of nitrate reductase by sparging CO for one minand with defined C0:02 ratios of 5 and 2.5. Effect of 2,4-DNP on CO-reaction in CO-insensitive CMS lines of sorghum at defined 0:02 ratio of 2.5 was also monitored. Separate experimentswere conducted for examining the effects of gibberellin (GA)on alpha-amylase induction in de-embryonated seeds of sorghumand the effect of foliar sprays of GA on the height of sorghumseedlings.xviiIn CO-aerobic assay of nitrate reductase bysparging CO for one min through leaf segments, out of the 13lines tested, the three, CMS lines 42A, CK 60A, AKMS-3A and therestorer SPV-504 were found to be CO-sensitive; while the remaining 9 CMS lines viz., 2219A, 296A, M-35-1A, M-31-2A, 53A,104A. 116A, 117A and the maintainer 296B were CO-insensitive.CO-sensitivity of sorghum lines with definedC0:02 ratios indicated that the leaves of all the sorghum lines responded to CO gas at a very low CO.-Oo ratio of 2.5. Nitriteproduction enhanced as the CO.-On ratio was increased from 2.5 to 5, thereby indicating that the redox state of cytochrome a, wasinfluenced by the concentration of CO in the gas mixture,leading to the corresponding inhibition of cytochrome oxidase.The pre-treatment of leaves of sorghum CMS lines with 2,4-DNPmodified the redox state of cytochrome c oxidase rendering COinsensitivesorghum lines responsive to CO.All the newly developed sorghum CMS lines testedwere insensitive to GA by alpha-amylase induction method.However, these lines, when tested by foliar sprays of differentconcentrations of GA, were found to be sensitive as evidencedby an increase in the plant height over the control.|
|Appears in Collections:||Thesis|
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