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|Authors:||BHITE, BABASAHEB RAMCHANDRA|
|Advisor:||Chavan, Dr . J . K .|
|Title:||BIOCHEMICAL ANALYSIS OF PIGEONPEA (Cajanus ca jan L. Millsp) VARIETIES HAVING DIFFERENTIAL REACTION TO STERILITY MOSAIC DISEASE|
|Agrotags:||sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas|
|Abstract:||The objective of this investigation ;vas to study the variations in preformed and induced levels of certain biochemical compounds generally implicated in the disease and pest resistance, between susceptible or resistant cultivars of pigeonpea to sterility mosaic disease during different growth stages. The usceptible and resistant cultivars, four in each group, were grown in glass houseconditions.to study variations in preformed levels ofdifferent biochemical compounds while in a separateexperiment, all the plants were inoculated at 60 days ofgrowth stage and placed in field conditions to develop axi disease. The changes in levels of different biochemical compounds as a result of induction by virus infection were studied in these plants. The biochemical parametersincluded total phenolics, chlorogenic acid, activities of polyphenol oxidase and peroxidase, reducing suqars, freeamino acids and soluble proteins in the leaves. The mean levels of total phenolics and chlorogenic acid were found to be significantly higher in resistant cultivars as compared to susceptible types. The contents of total phenolics and chlorogenic acid were found to bethe highest at 20 days of sowing and was found to continuouslydecrease subsequently upto 60 days of crop growth. While the activities of peroxidase and polyphenol oxidase, reducing sugars, free amino acids and soluble proteins were found toba higher at early growth stage and decreased later upto 60 days in all the susceptible and resistant cultivars. Amongthe preformed levels of different ompounds, the contents oftotal phenolics and chlorogenic acid were found to be higherin resistant cultivars than in the susceptible types, whilethe differences in activities of oxidative enzymes, reducingsugars, free amino adids and soluble proteins among the susceptible and resistant cultivars were not of significant er.Artificial inoculation of the plants at 60 days ofsowing markedly induced the activities of polyphenol oxidaseand peroxidase only in susceptible cultivars while itsignificantly increased the levels of reducing sugars andfree amino acids in both susceptible and resistantcultivars. Artificial inoculation of the plants howevereither did not induce or had no effects on the accumulationof chlorogenic acid and soluble protains in both susceptibleand resistant cultivars. The results of this preliminary studyindicated that preformed levels of total phenolics in generaland chlorogenic acid in particular might play an important role in imparting resistance to s t e r i l i t y mosaic diseasein pigeonpea. Further, studies are however essential toconfirm these findings.|
|Appears in Collections:||Thesis|
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