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Authors: Mahajan, Prasad N
Advisor: Munjal, Dr.S.V
Desai, Dr.B.B
Kute, Prof.L.S
Aher, Prof. B.P
Publisher: Mahatma Phule Krishi Vidyapeeth, Rahuri - 413 722, Dist. Ahmednagar, Maharashtra State (INDIA)
Language: en
Type: Thesis
Agrotags: sowing, intercropping, wheats, tillage equipment, seed drilling, crops, mustard, fertilizers, land resources, chickpeas
Abstract: The present investigation aims at evaluating the proximate composition of grains of twelve amaranth cultivars. In addition, the nitrate reductase (NR) activity from the leaves of amaranth plants was standardized by an in vivo method. The genetic potential of NR activity in twelve amaranth cultivars was also estimated. Further, it was desired to know the distribution of NR activity in different plant parts such as leaves, stems, petioles and roots in 60-day-old amaranth. The amaranth seedlings were grown in soil-filled pots under normal day light conditions at the Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri during the months from August to December, 1997. XV Abstract contd.... Mahajan P.N. The proximate composition indicated that crude protein content of the grains of amaranth cultivars varied from 12.34 to 16.86 per cent. The crude fat content rangedfrom 4.06 to 6.38 per cent, while tho crude fibre content varied from 2.90 to 4.34 per cent. The total ash content in the amaranth grains ranged from 1.97 to 2.93 per cent. The variation in carbohydrates content, determined by difference, was from 63.13 to 68.78 per cent. Calcium and phosphorus contents of the amaranth grains varied widely from 127.63 to 203.20 mg/100 g and 310.33 to 470.00 mg/100 g respectively. The iron content of the grains also showed a wide variation from 8.58 to 17.00 mg/100 g. Thus, the proximate composition of the amaranth grains reveals that the grains are the major source of proteins and minerals, viz., calcium and iron. The NR activity in amaranth leaves was determined by an in vivo method and the standardized infiltration media (5.0 ml) for determination of NR activity/in amaranth leaves included : nitrate, 100 mM; n-propanol (1 %, v/v) and Triton-X-100 (0.05 %, v/v) together with 100 mM Na-Pi buffer (pH 7.5). The enzyme NR exhibited diurnal rythms in amaranth leaves. The average NRactivity which was low at 7.00 a.m. in the morning increased rapidly as the day advanced until it reached a maximum level at 1.00 p.m. The NR activity, however, declined during the afternoon hours, reaching a minimum level in the evening atXVI Abstract contd.... Mahajan P.N. 7.00 p.m. The evaluation of the potential of the NR enzyme exhibited the highest NR activity in the cultivar Rasana-2 while the lowest was in AG-67. The significant correlation between NR activity in leaves and grain protein content of the twelve amaranth cultivars indicated that the cultivars with higher protein and NR activity such as AG-114 and Rasana-2 could be selected for breeding high protein graingenotypes. The highest NR activity in 60-day-old amaranthplants was located in petioles, indicating that petioles ace the major site of nitrate assimilation in amaranth, followed by leaf, stem and root in the decreasing order.
Subject: Agricultural Biochemistry
Theme: Agricultural Biochemistry
These Type: M.Sc
Issue Date: 1998-03-03
Appears in Collections:Thesis

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